MIA Protein (Transcript Variant 1)
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- Target Alle MIA Proteine anzeigen
- MIA (Melanoma Inhibitory Activity (MIA))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- Transcript Variant 1
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Applikation
- Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
- Spezifität
- Optimal preservation of protein structure, post-translational modifications and functions.
- Produktmerkmale
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- Recombinant human MIA (transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Reinheit
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin-Niveau
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- ED50 was determined by the dose-dependant proliferation of the human A375 melanoma cell line. The expected ED50 for this effect is 4-6ug/mL.
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- Applikationshinweise
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Handhabung
- Resuspend the protein in the desired concentration in proper buffer
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- MIA (Melanoma Inhibitory Activity (MIA))
- Andere Bezeichnung
- Mia (MIA Produkte)
- Hintergrund
- Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas. [UniProtKB/Swiss-Prot Function]
- Molekulargewicht
- 12.2 kDa
- NCBI Accession
- NP_006524
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