IL27 Protein

Produktnummer ABIN2723689

Produktdetails

Target: IL27 (Interleukin-27 Subunit alpha (IL27))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: HEK-293 Cells

Applikation: Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)

Spezifität: Optimal preservation of protein structure, post-translational modifications and functions.

Produktmerkmale:

• Recombinant human Interleukin-27 / IL27A protein expressed in HEK293.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Reinheit: Purity is > 95 % , as determined by Coomassie stained SDS-PAGE.

Endotoxin-Niveau: Less than 0.01 ng per μg protein as determined by the LAL method.

Biological Activity Comment: The ED50 is 5 - 10 ng/ml, corresponding to a specific activity of 1.0 - 2.0 x 10^5 units/mg, determined by inhibition of IL-2 production induced by mouse IL-27 in mouse splenocytes activated with anti-CD3 and anti-CD28 antibodies.

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: > 50 μg/mL

Buffer: 20 mM NaH2PO4, pH 6, 0.60 M NaCl

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: IL27 (Interleukin-27 Subunit alpha (IL27))

Andere Bezeichnung: Interleukin-27,il27a (IL27 Produkte)

Molekulargewicht: 49 kDa

NCBI Accession: NP_663611