Insulin-Degrading Enzyme (IDE) protein (Myc-DYKDDDDK Tag)

Details zu Produkt Nr. ABIN2723516, Anbieter: Anmelden zum Anzeigen
Proteinname
  • zgc:162603
  • IDE
  • 1300012G03Rik
  • 4833415K22Rik
  • AA675336
  • AI507533
  • INSDEGM
  • INSULYSIN
  • insulin degrading enzyme
  • insulin-degrading enzyme
  • metallopeptidase
  • IDE
  • ide
  • LOC591315
  • CC1G_01115
  • Bm1_26615
  • PTRG_10875
  • SJAG_01430
  • EBI_21700
  • VDBG_06702
  • LOAG_08082
  • PGTG_04982
  • Tsp_00424
  • Ide
Spezies
Human
10
3
1
Quelle
HEK-293 Cells
6
2
2
2
1
Protein-Typ
Recombinant
Aufreinigungstag / Konjugat
Myc-DYKDDDDK Tag
Applikation
Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Spezifität Optimal preservation of protein structure, post-translational modifications and functions.
Produktmerkmale
  • Recombinant human Insulin-degrading enzyme protein expressed in HEK293 cells.
  • Produced with end-sequenced ORF clone
Reinheit > 80 % as determined by SDS-PAGE and Coomassie blue staining
Proteinname
Hintergrund This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.[provided by RefSeq, Sep 2009].
Molekulargewicht 117.8 kDa
NCBI Accession NP_004960
Applikations-hinweise Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays
Kommentare

The tag is located at the C-terminal.

Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration > 50 μg/mL
Buffer 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
Lagerung -80 °C
Informationen zur Lagerung Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
Bilder des Herstellers
Western Blotting (WB) image for Insulin-Degrading Enzyme (IDE) protein (Myc-DYKDDDDK Tag) (ABIN2723516) Validation with Western Blot
Produkt verwendet in: Kim, Lone, Saghatelian: "Analysis of the proteolysis of bioactive peptides using a peptidomics approach." in: Nature protocols, Vol. 8, Issue 9, pp. 1730-42, 2013 (PubMed).

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