ATP6V0E1 Protein (Myc-DYKDDDDK Tag)

Produktnummer ABIN2715054

Produktdetails

Target: ATP6V0E1 (ATPase, H+ Transporting, Lysosomal 9kDa, V0 Subunit E1 (ATP6V0E1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses ATP6V0E1 Protein ist gelabelt mit Myc-DYKDDDDK Tag.

Applikation: Antibody Production (AbP), Standard (STD)

Produktmerkmale:

• Recombinant human ATP6V0E1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: ATP6V0E1 (ATPase, H+ Transporting, Lysosomal 9kDa, V0 Subunit E1 (ATP6V0E1))

Andere Bezeichnung: Atp6v0e1 (ATP6V0E1 Produkte)

Synonyme: ATP6H Protein, ATP6V0E Protein, M9.2 Protein, Vma21 Protein, Vma21p Protein, Atp6k Protein, Atp6v0e1 Protein, Atp6v0e Protein, Dsr-1A Protein, dsr-1 Protein, ATPase H+ transporting V0 subunit e1 Protein, ATPase, H+ transporting, lysosomal V0 subunit E Protein, ATP6V0E1 Protein, Atp6v0e Protein, Atp6v0e1 Protein

Hintergrund: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is possibly part of the V0 subunit. Since two nontranscribed pseudogenes have been found in dog, it is possible that the localization to chromosome 2 for this gene by radiation hybrid mapping is representing a pseudogene. Genomic mapping puts the chromosomal location on 5q35.3.

Molekulargewicht: 9.2 kDa

NCBI Accession: NP_003936

Pathways: Transition Metal Ion Homeostasis, Proton Transport