ATP Synthase Subunit gamma (AtpC) protein (His tag)

Produktnummer ABIN2715034

Produktdetails

Target: ATP Synthase Subunit gamma (AtpC)

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: His tag

Applikation: Antibody Production (AbP), Standard (STD)

Produktmerkmale:

• Recombinant human ATP synthase subunit gamma (full length, N-term HIS tag, transcript variant 2) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Kommentare:

The tag is located at the N-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: ATP Synthase Subunit gamma (AtpC)

Andere Bezeichnung: Atp Synthase Subunit gamma (AtpC Produkte)

Synonyme: ATPG_DROME Protein, ATPsyn-gam Protein, CG7610 Protein, Dmel\\CG7610 Protein, gamma subunit of Mt ATP synthase Protein, T21L14.5 Protein, ATP synthase, gamma subunit Protein, gamma subunit of Mt ATP synthase Protein, ATP synthase subunit gamma Protein, heat shock transcription factor, Y-linked 1-like Protein, ATPsyngamma Protein, ATP3 Protein, Desac_2456 Protein, LOC781463 Protein

Hintergrund: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha(3)beta(3). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. [UniProtKB/Swiss-Prot Function]

Molekulargewicht: 30 kDa

NCBI Accession: NP_005165

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process