Acetylcholinesterase Protein (AChE) (Myc-DYKDDDDK Tag)

Produktnummer ABIN2714318

Produktdetails

Target: Acetylcholinesterase (AChE)

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses Acetylcholinesterase Protein ist gelabelt mit Myc-DYKDDDDK Tag.

Applikation: Antibody Production (AbP), Standard (STD)

Produktmerkmale:

• Recombinant human Acetylcholinesterase (transcript variant E4-E5) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Reinheit: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Anwendungsinformationen

Applikationshinweise: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Kommentare:

The tag is located at the C-terminal.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Antigendetails

Target: Acetylcholinesterase (AChE)

Andere Bezeichnung: Acetylcholinesterase (AChE Produkte)

Synonyme: ACEE Protein, ARACHE Protein, N-ACHE Protein, YT Protein, Ache Protein, GB14873 Protein, zgc:92550 Protein, ACE Protein, Dsim\\GD20515 Protein, GD20515 Protein, dsim_GLEANR_4292 Protein, ache Protein, mE1a Protein, mE1b Protein, mE1c Protein, mE1c-long Protein, mE1d Protein, mE1d' Protein, mE1e Protein, arache Protein, n-ache Protein, acetylcholinesterase (Cartwright blood group) Protein, acetylcholinesterase 2 Protein, acetylcholinesterase Protein, Acetylcholine esterase Protein, acetylcholinesterase (Cartwright blood group) L homeolog Protein, collagen type I alpha 2 chain Protein, ACHE Protein, AChE-2 Protein, Ache Protein, ache Protein, Dsim\Ace Protein, ache.L Protein, COL1A2 Protein, ACE-1 Protein

Hintergrund: Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally.

Molekulargewicht: 60.8 kDa

NCBI Accession: NP_056646

Pathways: Skeletal Muscle Fiber Development