PRMT5 Protein (DYKDDDDK Tag)

Produktnummer ABIN2669658

Recombinant PRMT5-Protein exprimiert in Baculovirus.

Kurzübersicht für PRMT5 Protein (DYKDDDDK Tag) (ABIN2669658)

Target: PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Baculovirus

Applikation: Enzyme Activity Assay (EAA), Screening Assay (ScA)

Produktdetails

Aufreinigungstag / Konjugat: Dieses PRMT5 Protein ist gelabelt mit DYKDDDDK Tag.

Produktmerkmale: Recombinant PRMT5 (accession number NP_006100.2) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 74.2 kDa.

Anwendungsinformationen

Applikationshinweise: Recombinant PRMT5 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Specific Activity: H3R8 and H4R3 methyltransferase. HMT Assay Conditions:50 mM TrisCl, pH 8.6, 0.02 % Triton X-100, 2 mM MgCl2, 1 mM TCEP, 60 ng/μl H3 (1-21) peptide, 20 ng/μl Recombinant PRMT5 protein at 2 hours at room temperature. Activity was detected by fluorography.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Antigendetails

Target: PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))

Andere Bezeichnung: PRMT5

Hintergrund: PRMT5 (Protein Arginine Methyltransferase 5, also known as JBP1, Janus Binding Protein 1) is a type II arginine methyltransferase, a protein that transfers methyl groups to the arginine residues of substrate proteins. Arginine methylation is a common post-translational modification of histones and other cellular proteins. PRMT5 symmetrically dimethylates histone H2A Arg3, H3 Arg8 and H4 Arg3. It also methylates Piwi proteins which regulate small non-coding RNAs and snRNPs, regulating snRNP core particle assembly. PRMT5 functions as a co-repressor of transcription and is recruited by a number of DNA binding proteins and transcriptional repressor complexes. It is present both in the nucleus and the cytoplasm. It also interacts with COPR5, JAK2, PRDM1 and RPS10.

Molekulargewicht: 74.2 kDa

Pathways: Chromatin Binding, Regulation of Muscle Cell Differentiation, Ribonucleoprotein Complex Subunit Organization, Skeletal Muscle Fiber Development