HDAC4 Protein (AA 645-1057, Catalytic Domain) (His tag)

Produktnummer ABIN2669629

Dieses Recombinant HDAC4-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für HDAC4 Protein (AA 645-1057, Catalytic Domain) (His tag) (ABIN2669629)

Target: HDAC4 (Histone Deacetylase 4 (HDAC4))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Enzyme Activity Assay (EAA)

Produktdetails

Proteineigenschaft: Catalytic Domain, AA 645-1057

Aufreinigungstag / Konjugat: Dieses HDAC4 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant HDAC4 protein

Produktmerkmale: HDAC4 is a Class II histone deacetylase with broad substrate specificity. The catalytic domain corresponding to amino acids 645-1057 of HDAC4 (accession number NP_006028.2) was expressed in E. coli with an apparent molecular weight of 45 kDa. An epitope tag present during expression was removed proteolytically after purification. HDAC4 (Histone Deacetylase 4) is a member of the class IIa mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and 10), class III (SIRT1-7) and class IV (HDAC11). Unlike other deacetylases, HDAC4 shuttles between the nucleus and cytoplasm and serves as a nuclear co-repressor that regulates bone and muscle development. HDAC4 interacts with the myocyte enhancer factors Mef2a, Mef2c and Mef2d. It also forms part of a multi-protein complex with RbAp48 and HDAC3. HDAC4 is ubiquitous. Recombinant HDAC4 can be used in histone deacetylase (HDAC) assays.

Anwendungsinformationen

Applikationshinweise: Recombinant HDAC4 is suitable for use in histone deacetylase (HDAC) assays. A good starting point is 2 to 5 ng of enzyme per assay.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 μg of recombinant HDAC4 protein expressed in E. coli and provided in 25 mM HEPES pH 7.5, 200 mM KCl, 1 mM DTT and 50 % glycerol.

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Antigendetails

Target: HDAC4 (Histone Deacetylase 4 (HDAC4))

Andere Bezeichnung: HDAC4

Hintergrund:

Protein Background: HDAC4 (Histone Deacetylase 4) is a member of the class IIa mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-e-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and 10), class III (SIRT1-7) and class IV (HDAC11). Unlike other deacetylases, HDAC4 shuttles between the nucleus and cytoplasm and serves as a nuclear co-repressor that regulates bone and muscle development. HDAC4 interacts with the myocyte enhancer factors Mef2a, Mef2c and Mef2d. It also forms part of a multi-protein complex with RbAp48 and HDAC3. HDAC4 is ubiquitous.

Short Description: HDAC4 is a Class II histone deacetylase with broad substrate specificity. The catalytic domain corresponding to amino acids 645 - 1057 of HDAC4 (accession number NP_006028.2) was expressed in E. coli with an apparent molecular weight of 45 kDa. An epitope tag present during expression was removed proteolytically after purification.

Aliases: HD4, AHO3, BDMR, HDACA, HA6116, HDAC-4, HDAC-A

Molekulargewicht: 45 kDa

NCBI Accession: NP_006028

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process