Histone H3.1 Protein (HIST1H3B) (full length)

Produktnummer ABIN2669580

Produktdetails

Target: Histone H3.1 (HIST1H3B)

Protein-Typ: Recombinant

Proteineigenschaft: full length

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Enzyme Activity Assay (EAA), Screening Assay (ScA)

Produktmerkmale: Recombinant Histone H3.1, Human, corresponds to the native histone sequence of mammalian histone H3 variant H3.1 and does not contain any amino acid substitutions or residue analogs. Histone H3.1 is known to be important for gene regulation. Recombinant Histone H3.1, Human consists of the full-length sequence of human Histone H3.1 variant (accession number NM_003529). Recombinant Histone H3.1, Human was generated in E. coli cells and has an observed molecular weight of 15.4 kDa.

Anwendungsinformationen

Applikationshinweise: Recombinant Histone H3.1, Human is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 2 μg/μL

Handhabung: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Antigendetails

Target: Histone H3.1 (HIST1H3B)

Andere Bezeichnung: Histone H3.1 (HIST1H3B Produkte)

Synonyme: H3 histone Protein, Hist1h2ai Protein, histone cluster 1, H2ai-like1 Protein, histone H3.1 Protein, Hht21p Protein, Hist1h2ail1 Protein, H3.1 Protein, HHT21 Protein

Hintergrund: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points. Histone H3.1 and Histone H3.3 are the two main Histone H3 variants found in plants and animals. They are known to be important for gene regulation. Histone H3.1 and H3.3 have been shown to demonstrate unique genomic localization patterns thought to be associated with their specific functions in regulation of gene activity. Specifically, Histone H3.1 localization is found to coincide with genomic regions containing chromatin repressive marks (H3K9me3, H3K27me3 and DNA methylation), whereas Histone H3.3 primarily colocalizes with marks associated with gene activation (H3K4me3, H2BK120ub1, and RNA pol II occupancy). Deposition of the Histone H3.1 variant into the nucleosome correlates with the canonical DNA synthesis-dependent deposition pathway, whereas Histone H3.3 primarily serves as the replacement Histone H3 variant outside of S-phase, such as during gene transcription. Aberrant localization of these variants is also known to correlate with certain cancers.

Molekulargewicht: 15.4 kDa