Histone H3.2 (biotinylated), (full length), (N-Term), (truncated) Protein

Produktnummer ABIN2669559

Recombinant -Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für Histone H3.2 (biotinylated), (full length), (N-Term), (truncated) Protein (ABIN2669559)

Target: Histone H3.2

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Positive Control (PC), Substrate (S)

Reinheit: Protein was determined to have ≥ 98 % purity by SDS-PAGE.

Produktdetails

Proteineigenschaft: N-Term, truncated, biotinylated, full length

Produktmerkmale: Truncated human Histone H3.2 is produced in E. coli and purified using FPLC. The purified protein is subsequently ligated to a peptide containing the histone tail with a N-terminal biotin via a native peptide bond. The full-length protein is then repurified prior to lyophilization. Protein concentration was determined using the molar extinction coefficient for Histone H3 and absorbance at 280nm. Protein was determined to have ≥ 98 % purity by SDS-PAGE. The molecular weight is 15,565 Daltons.

Aufreinigung: Purified using FPLC

Anwendungsinformationen

Applikationshinweise: Recombinant histones are suitable for use as positive controls in the analysis of histone post-translational modifications, as substrates for histone modification enzymes, or to generate chromatin in vitro.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recombinant histones can be resuspended in water or any suitable buffer. We recommend a starting concentration of 1 mg/mL. To fully solubilize the histone we suggest resuspension in the buffer of choice at room temperature for 20-30 minutes with occasional pipetting. Addition of salt or Tris to the resuspension buffer may enhance histone solubility.

Handhabung: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Antigendetails

Target: Histone H3.2

Hintergrund: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points.

Molekulargewicht: The molecular weight is 15,565 Daltons.