Histone H4 Protein (meLys5)

Produktnummer ABIN2669553

Recombinant Histone H4-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für Histone H4 Protein (meLys5) (ABIN2669553)

Target: Histone H4

Protein-Typ: Recombinant

Spezies: Xenopus laevis

Quelle: Escherichia coli (E. coli)

Applikation: Positive Control (PC), Substrate (S)

Reinheit: The recombinant histone is >98 % pure by SDS-PAGE.

Produktdetails

Proteineigenschaft: meLys5

Produktmerkmale: Recombinant Xenopus laevis Histone H4 monomethyl Lys5 (H4K5me1) is produced in E. coli and purified using FPLC. Recombinant methylated histones are specifically methylated via a chemical alkylation reaction that introduces a methyl lysine analog (MLA). This specific chemical treatment enables the site and degree of methylation to be controlled precisely. Each methylation reaction is over 99 % complete, as verified by high-resolution ESI-TOF mass spectrometry. Protein concentration was determined using the molar extinction coefficient for Histone H4 and absorbance at 280nm. The recombinant histone is >98 % pure by SDS-PAGE. The molecular weight of the recombinant histone is 11,268 Daltons.

Aufreinigung: Purified using FPLC

Anwendungsinformationen

Applikationshinweise: Recombinant histones are suitable for use as positive controls in the analysis of histone post-translational modifications, as substrates for histone modification enzymes, or to generate chromatin in vitro.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recombinant histones can be resuspended in water or any suitable buffer. We recommend a starting concentration of 1 mg/mL. To fully solubilize the histone we suggest resuspension in the buffer of choice at room temperature for 20-30 minutes with occasional pipetting. Addition of salt or Tris to the resuspension buffer may enhance histone solubility.

Handhabung: Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.

Antigendetails

Target: Histone H4

Hintergrund: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points.

Molekulargewicht: The molecular weight of the recombinant histone is 11,268 Daltons.