IL17F Protein (AA 31-163)

Produktnummer ABIN2667479

Dieses Recombinant IL17F-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für IL17F Protein (AA 31-163) (ABIN2667479)

Target: IL17F (Interleukin 17F (IL17F))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Reinheit: >98 % , as determined by Coomassie stained SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 31-163

Sterilität: 0.22 μm filtered

Endotoxin-Niveau:

Less than 0.01ng per μg cytokine as determined by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: ED50 =400 - 800 ng/ml, corresponding to a specific activity of 2.5 - 1.25 x 103 units/mg, as determined by the dose dependent stimulation of CXCL1 production in human fibroblast.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in PBS

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Antigendetails

Target: IL17F (Interleukin 17F (IL17F))

Andere Bezeichnung: IL-17F

Hintergrund: IL-17F belongs to the IL-17 cytokine family which includes IL-17A, B, C, D, and E (also called IL-25). IL-17F shares the strongest homology to IL-17A. They share 50 % amino acid sequence homology. The genes encoding IL-17 and IL-17F are localized in the same chromosomal region and are co-expressed by CD4+ and gamma delta T cells. Recently, an IL-17-IL-17F heterodimer was found to be expressed in Th17 cells together with IL-17 and IL-17F homodimers. Similar to IL-17, IL-17F utilizes IL-17RA and IL-17RC as its receptor and employs Act1 and TRAF6 as its signal transducers to induce the expression of pro-inflammatory cytokines and chemokines in many different cell types. IL-17F expression is upregulated in inflammatory bowel disease. A His161 to Arg161 (H161R) substitution in the third exon of the IL17F gene seems to be associated with asthma and chronic obstructive pulmonary disease (COPD) in Japanese subjects. In addition polymorphism of IL-17F seems to be associated to susceptibility to gastric cancer.

Molekulargewicht: The 133 amino acid recombinant protein has a predicted molecular mass of 14903.1 Da. This protein exists as a disulfide-linked homodimer. The DTT-reduced protein migrates at approximately 15kDa by SDS-PAGE. The non-reduced protein migrates as a homodimer,

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity