Interleukin 17a Protein (AA 20-155)

Produktnummer ABIN2667470

Dieses Recombinant Interleukin 17a-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für Interleukin 17a Protein (AA 20-155) (ABIN2667470)

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: ELISA, Biochemical Assay (BCA)

Reinheit: Purity is >98 % as determined by SDS PAGE, Coomassie stained with Invitrogen SimplyBlue SafeStain.

Produktdetails

Proteineigenschaft: AA 20-155

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.,Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Prior to use, reconstitute the lyophilized powder with 0.2 mLof PBS containing a carrier protein (e.g., 1 % BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70 °C for up to one month.

Buffer: Lyophilized in sterile-filtered PBS, pH 7.2, containing 1 % BSA.

Handhabung: Use a manual defrost freezer and avoid repeated freeze thaw cycles.

Lagerung: 4 °C

Informationen zur Lagerung: Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt.

Haltbarkeit: 12 months

Antigendetails

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Andere Bezeichnung: IL-17A

Hintergrund: IL-17A is the founding member of the IL-17 family, a group of six structurally related pro-inflammatory cytokines. IL-17A, secreted by activated CD4+ Th17 cell subpopulation, elicits multiple biological activities on a variety of cells including: the induction of IL-6, IL-8, G-CSF, and PGE2 production in epithelial, endothelial or fibroblasts, the enhancement of surface expression of ICAM-1 in fibroblasts, activation of NF-κB and costimulation of T cell proliferation. Recent studies demonstrated that, in mice, activated IL-17-secreting CD4+ helper T cells (Th17 cells)mediate an autoimmune arthritis that clinically and immunologically resembles rheumatoid arthritis (RA).Human IL-17A shows 63 % , 63 % and 72 % amino acid sequence identity to rat IL-17A, mouse IL-17A and a protein encoded by the ORF13 gene of herpesvirus Saimiri (HVS), respectively.Both recombinant and natural IL-17A have been shown to exist as a disulfide-linked homodimeric glycoprotein containing two ~16 kD monomers.

Molekulargewicht: The 137 amino acid N-terminal methionylated protein has a predicted molecular mass of 15,666 Da. This protein exists as a disulfide-linked homodimer. The DTT-reduced protein migrates at approximately 16kDa by SDS-PAGE. The non-reduced protein migrates