IL-25 Protein (AA 17-169)

Produktnummer ABIN2666841

Recombinant IL-25-Protein exprimiert in Insektenzellen.

Kurzübersicht für IL-25 Protein (AA 17-169) (ABIN2666841)

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Insektenzellen

Applikation: Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Reinheit: > 95 % , as determined by Coomassie stained SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 17-169

Sterilität: 0.22 μm filtered

Endotoxin-Niveau:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: Mouse IL-25 binds to recombinant mouse IL-17BR/Fc chimera in a functional ELISA.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Buffer: 0.22 μm filtered protein solution is in 20 mM NaHPO4 pH 6.0, 0.5 M NaCl.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Antigendetails

Target: IL-25 (IL25) (Interleukin 25 (IL25))

Andere Bezeichnung: IL-25

Hintergrund: IL-25 (also known as IL-17E) is a member of the IL-17 family, which includes IL-17A, IL-17B, IL-17C, IL-17F, and IL-17A/F. The primary sequence homology differs between family members, with mouse IL-17A and IL-17F having the highest homology (49 % ), while mouse IL-17A and IL-25 have the lowest (28 %). IL-25 induces gene expression of IL-4, IL-5, and IL-13 in multiple tissues, and this induction results in T helper 2 (TH2)-type immune responses, this is characterized by an increase in serum IgE levels, pathologic changes in the lungs and digestive tract with eosinophilic infiltrates, an increase in mucus production, epithelial cell hyperplasia, and overall amplified allergic inflammation. IL-25 shares the receptor IL-17RB with IL-17B, although it binds with much higher affinity than IL-17B.

Molekulargewicht: The 153 amino acid recombinant protein has a predicted molecular mass of 17.6 kDa. This protein exists as a disulfide-linked homodimer. The DTT-reduced protein migrates at approximately 20 kDa by SDS-PAGE. The non-reduced protein migrates as a homodimer,