CCL20 Protein (AA 26-96)

Produktnummer ABIN2666785

Dieses Recombinant CCL20-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für CCL20 Protein (AA 26-96) (ABIN2666785)

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Ratte

Quelle: Escherichia coli (E. coli)

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Reinheit: > 95 % , as determined by Coomassie stained SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 26-96

Sterilität: 0.22 μm filtered

Endotoxin-Niveau:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: Bioactivity was measured by its property to chemoattract Baf3-hCCR6 transfectants in a dose dependent manner.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Buffer: 0.22 μm filtered protein solution is in PBS.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Antigendetails

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Andere Bezeichnung: CCL20

Hintergrund: Rat CCL20 was initially identified in ischemic brain tissue and called ST38. In experimental autoimmune panencephalomyelitis, CCL20 expression correlates with the onset of inflammation. In experimental autoimmune encephalomyelitis (EAE), CCL20 and CCR6 mRNAs are induced in the CNS of SJL mice, CCL20 is expressed mainly by CNS-infiltrating leukocytes, and it can be expressed by astrocytes as an additional source of CCL20 during disease relapses. CCL20 is involved in the recruitment of leukocytes to sites of injury and inflammation. CCL20 is constitutively expressed in liver, lung, and mucosal epithelial cells (small intestine and colon). CCL20 is expressed at a low basal level, but its expression is amplified by proinflammatory cytokines and toll-like receptor (TLR) agonists. During infections, CCL20 expression is stimulated in epithelial cells, and immature CCR6+ dendritc cells (DCs) are recruited. Thereafter, the CCR6 expression is downregulated during DC maturation and these migrate to the lymphoid node under the expression of CCR7. CCL20 is the only ligand for CCR6. Nevertheless, mouse and human β -defensin-1 and -2, small cationic antimicrobial peptides, bind and activate CCR6. Contrarily, these β-defensins have lower affinities for CCR6 than CCL20.

Molekulargewicht: The 71 amino acid recombinant protein has a predicted molecular mass of approximately 8.2 kDa. The DTT-reduced protein migrates at approximately 8 kDa and non-reduced protein at approximately 10 kDa by SDS-PAGE. The N-terminal amino acid is Ala.

Pathways: Phosphorylierungen bei SARS-CoV-2 Infektion