Lipocalin 2 Protein (LCN2) (AA 21-198, C-Term, His10)

Produktnummer ABIN2666721

Recombinant Lipocalin 2-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für Lipocalin 2 Protein (LCN2) (AA 21-198, C-Term, His10) (ABIN2666721)

Target: Lipocalin 2 (LCN2)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Applikation: Western Blotting (WB)

Reinheit: > 95 % , as determined by Coomassie stained SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 21-198, His10, C-Term

Sterilität: 0.22 μm filtered

Endotoxin-Niveau:

Less than 0.01 ng per μg cytokine as determine by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: BC50 ≥ 1.2 μM, by dose dependent binding of the protein with [ Fe3+(DHBA)3] complex.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For maximum results, quick spin vial prior to opening.

Buffer: 0.22 μm filtered protein solution is in PBS.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for three months, or at -70°C for six months.

Antigendetails

Target: Lipocalin 2 (LCN2)

Andere Bezeichnung: NGAL

Hintergrund: NGAL, also known as lipocalin 2, LCN2, and siderocalin, is a small molecule composed of 178 amino acids. It belongs to the lipocalin superfamily of 20 structurally related secreted proteins. Members of the lipocalin family share a highly conserved fold with an eight stranded antiparallel β barrel, and act as a transporter, carrying small molecules to specific cells. NGAL occurs predominantly in a monomeric form, with a small percentage occurring as a dimer or trimer. It was originally identified as a 25 kD protein covalently bound to gelatinase from human neutrophils. It is a critical component of innate immunity because its ability to bind iron-siderophore complexes limits bacterial growth. Human NGAL is expressed at very low levels in human tissues, including kidney, trachea, lungs, stomach, and colon, and its expression increases greatly in the presence of inflammation and injured epithelia. In addition to its role in innate immunity, it is a promising biomarker for acute kidney injury that is beginning to be used in clinical practice in addition to research studies.

Molekulargewicht: The 193 amino acid recombinant protein has a predicted molecular mass of approximately 22 kDa. The DTT-reduced and non-reduced protein migrate at approximately 24-28 and 23-26 kDa by SDS-PAGE respectively. The N-terminal amino acid is blocked.

Pathways: Cellular Response to Molecule of Bacterial Origin, Transition Metal Ion Homeostasis