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TIMP1 Protein (AA 27-205, C-Term)

TIMP1 Spezies: Maus Wirt: HEK-293 Cells Recombinant > 90 % , as determined by Coomassie stained SDS-PAGE. WB Active
Produktnummer ABIN2666718
  • Target Alle TIMP1 Proteine anzeigen
    TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Proteineigenschaft
    AA 27-205, C-Term
    Spezies
    • 11
    • 10
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Maus
    Quelle
    • 16
    • 10
    • 8
    • 4
    • 2
    • 1
    • 1
    • 1
    HEK-293 Cells
    Applikation
    Western Blotting (WB)
    Reinheit
    > 90 % , as determined by Coomassie stained SDS-PAGE.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau

    Less than 1.0 EU per μg of protein as determine by the LAL method.

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    Discover our top product TIMP1 Protein
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Kommentare

    Biological activity: Mouse TIMP-1 inhibits the activity of activated mouse MMP-9 (100 ng/mL). The IC50 ≤ 90 ng/mL.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Rekonstitution
    For maximum results, quick spin vial prior to opening.
    Buffer
    0.22 μm filtered protein solution is in 20 mM Tris, 150 mM NaCl, pH 8.0.
    Handhabung
    Avoid repeated freeze/thaw cycles.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for three months, or at -70°C for six months.
  • Target
    TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))
    Andere Bezeichnung
    TIMP-1 (TIMP1 Produkte)
    Synonyme
    TIMP-1 Protein, TIMP1 Protein, DKFZp468A0912 Protein, CLGI Protein, EPA Protein, EPO Protein, HCI Protein, TIMP Protein, Timp Protein, Clgi Protein, TIMP metallopeptidase inhibitor 1 Protein, tissue inhibitor of metalloproteinase 1 Protein, TIMP1 Protein, Timp1 Protein
    Hintergrund
    TIMPs (tissue inhibitors of metalloproteinases) are endogenous inhibitors for MMPs (matrix metalloproteinases). The TIMPs consist of structurally and functionally distinct N- and C-terminal domains, each of which is stabilized by three disulfide bonds. The N-terminal domain binds to the active site of MMPs, inhibiting their proteolytic activities. The bidentate coordination of the zinc ion in the active site of an MMP by the N-terminal α-amino group and carbonyl group of the Cys residue in the "cysteine switch" is a key mechanism of inhibition with MMPs. The TIMP's C-terminal domain is known to bind hemopexin-like domain of pro-MMPs. TIMPs are broad-spectrum inhibitors of MMPs, but there are some differences in specificity among them. Mouse metalloproteinase inhibitor 1, TIMP-1, is more restricted in its inhibitory range than the other three TIMPs, having a relatively low affinity for the membrane-type MMPs, MMP-14, MMP-16, and MMP-24 as well as for MMP-19. Mouse TIMP-1 shares a 72 % homology with its human counterpart. It is widely expressed in many mammalian tissues, notably in the reproductive organs. TIMP-1 is able to promote cell proliferation in a wide range of cell types and may also have an anti-apoptotic function. It has anti-angiogenic activity by preventing endothelial cell migration. CD63 has been identified as a receptor for TIMP-1. TIMP-1 binding to CD63 inhibits apoptosis and arrests cell growth. TIMP-1-null mice exhibit a number of alterations in processes associated with reproduction, steroidogenesis and impaired learning and memory. In addition, mice deficient in TIMP-1 exhibit enhanced MMP activity that would contribute to a reduction of atherosclerotic plaque size, neverthless, it promotes aneurysm formation.
    Molekulargewicht
    Predicted molecular mass of approximately 22 kDa. The protein migrates at about 25 kDa in DTT-reducing conditions and about 22 kDa in non-reducing condition by SDS-PAGE. The N-terminal amino acid is Cys.
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