Thymic Stromal Lymphopoietin Protein (TSLP) (AA 29-159)

Produktnummer ABIN2666676

Recombinant Thymic Stromal Lymphopoietin-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für Thymic Stromal Lymphopoietin Protein (TSLP) (AA 29-159) (ABIN2666676)

Target: Thymic Stromal Lymphopoietin (TSLP)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Flow Cytometry (FACS)

Reinheit: > 98 % , as determined by Coomassie stained SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 29-159

Sterilität: 0.22 μm filtered

Endotoxin-Niveau:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: ED50 = 1 - 5 ng/ml, corresponding to a specific activity of 0.2 - 1 x 106 units/mg, as determined by the dose dependent stimulation of mouse BaF3-human IL-7a and human TSLPR cotransfectant cell proliferation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in 20 mM NaHPO4, pH 7.2, 0.15 M NaCl.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Antigendetails

Target: Thymic Stromal Lymphopoietin (TSLP)

Andere Bezeichnung: TSLP

Hintergrund: TSLP was initially identified in conditioned medium from a mouse thymic stromal cell line. Human and mouse TSLP are only 43 % identical at the amino acid level, and TSLP, like IL-7, exhibits species-specific functions and may function differently in humans and mice. TSLP binds to a heterodimeric receptor constituted by IL-7Ra and TSLPR. TSLPR has low affinity for TSLP, but in combination with IL-7Ra generates a high affinity binding site. TSLP has been associated with the development of allergic disorders in humans, such as asthma, atopic dermatitis, and food allergies. The susceptibility to allergic diseases has been associated to basophil induction by TSLP, and Th2 cytokine production induced by TSLP in mast cells. In addition, TSLP has been associated to the development of Tregs, and blocking TSLPR leads to delayed functional maturation of thymic Tregs. TSLPR has been detected in Tregs from allergic asthmatic patients. TSLP directly activates STAT5 in Tregs and suppresses its suppressive activities, additionally, it inhibits IL-10 production. Human TSLP induces the release of T cell-attracting chemokines (CCL17 and CCL22) from monocytes and enhances the T cell stimulatory capacity of the CD11c+ subset of dendritic cells. TSLP induces proliferation of naive T cells. In addition, it can drive Th2 differentiation via the induction of IL-4 production, and drives Th2 cytokine-mediated inflammation by acting on bone marrow-resident progenitors, which promotes basophil hematopoiesis. TSLP is upregulated in epithelial cells by IL-1α, TNFα, and Th2 cytokines.

Molekulargewicht: The 132 amino acid recombinant protein has a predicted molecular mass of approximately 15 kDa. The DTT-reduced protein migrates at approximately 17 kDa and non-reduced protein at approximately 18 kDa by SDS-PAGE. The N-terminal amino acid is Met.