CXCL2 Protein (AA 28-100)

Produktnummer ABIN2666642

Dieses Recombinant CXCL2-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für CXCL2 Protein (AA 28-100) (ABIN2666642)

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: Multiplex Assay (MA)

Reinheit: > 98 % , as determined by Coomassie stained SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 28-100

Sterilität: 0.22 μm filtered

Endotoxin-Niveau:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: Bioactivity was measured by its property to chemoattract human neutrophils in a dose dependent manner.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in PBS.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Antigendetails

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Andere Bezeichnung: CXCL2

Hintergrund: CXCL2 is an ELR CXC chemokine, and it is structurally and functionally related to GRO1 (CXCL1), GRO3 (CXCL3), and interleukin-8 (CXCL8). CXCL2 binds to the CXCR2 receptor, and this receptor is shared with other ELR CXC chemokines (CXCL1, CXCL3, CXCL5, CXCL6, CXCL7, and CXCL8). CXCL2 is highly expressed in esophageal squamous cell carcinoma (ESCC), and it has been detected in serum of patients with this disease. Also, CXCL1, CXCL2, and CXCL3 have been shown to be highly expressed in patients with malignant melanoma. In addition, CXCL2 and CXCL1 are up-regulated in the central nervous system in multiple sclerosis (MS) and in the animal model of experimental autoimmune encephalomyelitis (EAE). In EAE, it has been shown that transfer of encephalitogenic CD4+ Th17 cells induce CXCL1 and CXCL2 transcription in the spinal cords of naive, syngeneic recipients. IL-17 is a potent inducer of ELR+ CXC chemokines in MS and EAE. In fact, it has been showed that CXCL1 and CXCL2 are up-regulated in the preclinical and clinical stages of EAE. In addition, it has been shown that CXCL1 and CXCL2 are secreted by ephithelial cells in bone marrow, and CXCL2 is secreted by endothelial cells during G-CSF-induced neutrophil mobilization.

Molekulargewicht: The 73 amino acid recombinant protein has a predicted molecular mass of approximately 7.8 kDa. The DTT-reduced protein migrates at approximately 10 kDa and non-reduced protein migrates at 12 kDa by SDS-PAGE. The N-terminal amino acid is Alanine.

Pathways: Cellular Response to Molecule of Bacterial Origin