CCL20 Protein (AA 27-96)

Produktnummer ABIN2666608

Dieses Recombinant CCL20-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für CCL20 Protein (AA 27-96) (ABIN2666608)

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: Multiplex Assay (MA)

Reinheit: > 98 % , as determined by Coomassie stained SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 27-96

Sterilität: 0.22 μm filtered

Endotoxin-Niveau:

Less than 0.01 ng per μg cytokine as determined by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: Bioactivity was measured by its property to chemoattract Baf3-hCCR6 transfectants in a dose dependent manner.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored from -20 °C to -70 °C. Stock solutions can also be prepared at 50-100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.

Buffer: 0.22 μm filtered protein solution is in PBS.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.

Antigendetails

Target: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Andere Bezeichnung: CCL20

Hintergrund: CCL20 is an inflammatory cytokine involved in the recruitment of leukocytes to sites of injury and inflammation. CCL20 is expressed at a low basal level, but it is induced by proinflammatory cytokines and Toll-like receptor (TLR) agonists. During infections, CCL20 expression is stimulated in epithelial cells, and immature CCR6+ dendritic cells (DCs) are recruited. Thereafter, CCR6 is downregulated during DC maturation, and the DCs migrates to the lymph node under the expression of CCR7. CCL20 is the only ligand for CCR6. Nevertheless, mouse and human β -defensin-1 and -2, small cationic antimicrobial peptides, bind and activate CCR6. Contrarily, these β-defensins have lower affinities for CCR6 than CCL20. In fact, human CCL20 has potent antimicrobial activity.

Molekulargewicht: The 70 amino acid recombinant protein has a predicted molecular mass of approximately 8.0 kDa. The DTT-reduced protein migrates at approximately 11 kDa and non-reduced protein at approximately 12 kDa by SDS-PAGE. The N-terminal amino acid is Ala.

Pathways: Phosphorylierungen bei SARS-CoV-2 Infektion