TNFSF14 Protein (AA 72-239, N-Term)
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- Target Alle TNFSF14 Proteine anzeigen
- TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 72-239, N-Term
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Spezies
- Maus
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Quelle
- CHO Cells
- Applikation
- Flow Cytometry (FACS)
- Reinheit
- > 95 % , as determined by Coomassie stained SDS-PAGE.
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
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Less than 0.01 ng per μg cytokine as determined by the LAL method.
- Top Product
- Discover our top product TNFSF14 Protein
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Kommentare
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Biological activity: ED50 = 1.0 - 5.0 μg/mL, as determined by cytotoxicity effect of HTâ€'29 human colon adenocarcinoma cells in the presence of human IFN-γ.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Rekonstitution
- For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20 °C to -70 °C. Stock solutions can also be prepared at 50 - 100 μg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2 - 1 % BSA or HSA and stored in working aliquots at -20 °C to -70 °C.
- Buffer
- 0.22 μm filtered protein solution is in PBS, pH 7.2.
- Handhabung
- Avoid repeated freeze/thaw cycles.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year.
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- Target
- TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))
- Andere Bezeichnung
- LIGHT (TNFSF14 Produkte)
- Synonyme
- TNFSF14 Protein, CD258 Protein, HVEML Protein, LIGHT Protein, LTg Protein, TR2 Protein, HVEM-L Protein, Ly113 Protein, TNF superfamily member 14 Protein, tumor necrosis factor (ligand) superfamily, member 14 Protein, TNFSF14 Protein, Tnfsf14 Protein
- Hintergrund
- LIGHT is a member of the TNF family which includes TNF-α, TNF-β (lymphotoxin-alpha, LT-α), CD40 ligand, TRAIL (Apo2L), Fas ligand (FASL), and osteoprotegerin (OPG) ligand. LIGHT is a type II transmembranal protein that possesses 34 % identity with lymphotoxin beta (LT-β) and 31 % identity with FASL. LIGHT stimulates the proliferation of activated HVEM+ T-cells, NF-κB activation, and induces apoptosis in cells expressing both HVEM and LT-β receptors. B- and T- lymphocyte attenuators (BTLA) also bind to HVEM. LIGHT, as well as other members of the TNF/TNFR family, is released from the membrane by MMPs. It plays a key role for optimal IL-12 production by the dendritic cells (DCs) and the development of IFN-γ-producing CD4+ Th1 cells. Bone marrow-derived DCs from LIGHT (-/-) mice are severely impaired in IL-12p40 production after IFN-γ and LPS stimulation in vitro. LIGHT is expressed in lung inflammatory leucocytes after an allergen house dust mite challenge. It induces airway remodeling via the induction of TGF-β. The inhibition of LIGHT, using a fusion protein of Fc with the extracellular portion of the LT-β receptor (LTβR-Fc), prevents LIGHT-LTβR and LIGHT-HVEM interaction. In a mouse model of chronic asthma, this reduces lung fibrosis, smooth muscle and epithelial hyperplasia, and airway hyperresponsiveness through the suppression of lung TGF-β and IL-13 production. LIGHT has been shown to increase the anti-tumor immune response and its overexpression is associated with the survival in patients with colorectal cancer liver metastasis.
- Molekulargewicht
- The 189 amino acid recombinant protein has a predicted molecular mass of approximately 20.5 kDa. The DTT-reduced and non-reduced protein migrate at approximately 30 and 28 kDa respectively by SDS-PAGE. The predicted N-terminal amino acid is His.
- Pathways
- Cancer Immune Checkpoints
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