MMP8 Protein (AA 21-467, C-Term)

Produktnummer ABIN2666511

Recombinant MMP8-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für MMP8 Protein (AA 21-467, C-Term) (ABIN2666511)

Target: MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Applikation: Flow Cytometry (FACS)

Reinheit: > 95 % , as determined by Coomassie stained SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 21-467, C-Term

Sterilität: 0.22 μm filtered

Endotoxin-Niveau:

Less than 1.0 EU per μg of protein as determined by the LAL method.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Biological activity: Activated human MMP-8 is able to cleave the peptide substrate Mca-KPLGL-Dpa-AR-NH2 with an activity above 375 pmol/min/μg.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: For maximum results, quick spin vial prior to opening.

Buffer: 0.22 μm filtered protein solution is in TCN (25 mM TRIS, 10 mM CaCl2, 150 mM NaCl, pH 7.5).

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Unopened vial can be stored at -70°C for six months.

Antigendetails

Target: MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

Andere Bezeichnung: MMP-8

Hintergrund: MMP-8, also termed neutrophil collagenase, is a member of the matrix metalloproteinase family proteins (MMPs). Members of this family are structurally related, zinc-containing enzymes that degrade the extracellular matrix (ECM) and connective tissue proteins. MMP-8 consists of a prodomain that is cleaved upon activation, a catalytic domain containing the zinc binding site, a hinge region, and a carboxyl terminal domain with hemopexin-like repeats. Substrates of MMP-8 include type I, II, and III triple-helical collagens, gelatin peptides, proteoglycans, fibronectin, aggrecan, substance P, serpins, β-casein, and angiotensin. MMP-8 is released by neutrophils upon IL-1β IL-8, GM-CSF and TNF-α stimulation. Besides playing a role in phagocytosis, MMP-8 has been implicated in rheumatoid arthritis (RA) because of its high efficiency in infiltrating connective tissue and breaking down ECM. In fact, serum level of MMP-8 is a strong predictor of mortality in RA, as this is consistent with increased activation of neutrophils in RA. In addition, MMP-8 has been shown to play a crucial role in cardiovascular diseases by promoting atherosclerotic lesion formation. This is supported by the finding that MMP-8 knockout mice have less endothelial cells or plaque angiogenesis in the atherosclerotic plaques. In a clinical study, it was shown that the ratio of MMP-9/TIMP-1, as well as MMP-8 serum levels, could be a useful predictor of prognosis in patients with hepatocellular carcinomas.

Molekulargewicht: This 460 amino acid recombinant protein has a predicted molecular mass of approximately 52.6 kDa. The protein migrates at approximately 70 kDa in DTT-reducing conditions and approximately 70 kDa in non-reducing conditions by SDS-PAGE.The predicted N-termi