TM Pst1 Protein

Produktnummer ABIN2469152

Dieses Recombinant -Protein wird in Yeast (Saccharomyces Cerevisiae) produziert.

Kurzübersicht für TM Pst1 Protein (ABIN2469152)

Target: TM Pst1

Protein-Typ: Recombinant

Spezies: Saccharomyces cerevisiae

Quelle: Yeast (Saccharomyces Cerevisiae)

Applikation: Screening Assay (ScA), ELISA, Western Blotting (WB)

Reinheit: ~95 %

Produktdetails

Anwendungsinformationen

Applikationshinweise: This recombinant protein can be used for WB, ELISA, and other assays to screen and identify mannose-specific antibodies.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Lot specific, TBS or PBS

Handhabung: Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Lagerung: -80 °C

Informationen zur Lagerung: Store in working aliquots at -70 °C.

Antigendetails

Target: TM Pst1

Hintergrund: Protoplast secreted protein 1 (Pst1) is an anchored plasma membrane protein. Pst1 was previously identified as a protein secreted by yeast regenerating protoplasts, which suggests a role in cell wall construction (1). Pst1 is a protein with 444 amino acids and is attached to yeast cell wall via a glycosylphosphatidylinisotol (GPI) anchor (2). Pst1 contains 15 potential N-linked glycosylation sites and is heavily glycosylated. It migrates at around 200 kDa on SDS-PAGE when produced in wild type S. cerevisiae. Our Pst1 protein was expressed in a genetically manipulated triple-mutant (TM) S. cerevisiae stain (?och1 ?mnn1 ?mnn4), which results in the production of sole Man8GlcNAc2 carbohydrate structures and Pst1 migration at approximately 100 kDa. When produced in the TM yeast, Pst1 can be recognized by several HIV-1 broadly neutralizing antibodies, including 2G12 and recently identified glycan-specific PGT antibodies (3). Among several heavily N-glycosylated TM yeast glycoproteins, Pst1 shows high affinity for 2G12 and efficiently inhibits gp120 interactions with 2G12 and DC-SIGN, and it also blocks 2G12-mediated neutralization of HIV-1 pseudoviruses (4).

Molekulargewicht: 100 kDa on SDS-PAGE

Gen-ID: 851625

OMIM: 17380259

UniProt: Q12355