Mycoplasma Arginine Deiminase (Active) Protein

Produktnummer ABIN2468872

Recombinant Mycoplasma Arginine Deiminase-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für Mycoplasma Arginine Deiminase (Active) Protein (ABIN2468872)

Target: Mycoplasma Arginine Deiminase

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Mycoplasma crocodyli

Quelle: Escherichia coli (E. coli)

Reinheit: < 97 % by SDS-PAGE gel and HPLC analyses.

Produktdetails

Sequenz: MSVFDSKFKG IHVYSEIGEL ESVLVHEPGR EIDYITPARL DELLFSAILE SHDARKEHKQ FVAELKANDI NVVELIDLVA ETYDLASQEA KDKLIEEFLE DSEPVLSEEH KVVVRNFLKA KKTSRELVEI MMAGITKTDL GIEADHELIV DPMPNLYFTR DPFASVGNGV TIHYMRYKVR QRETLFSRFV FSNHPKLINT PWYYDPSLKL SIEGGDVFIY NNDTLVVGVS ERTDLQTVTL LAKNIVANKE CEFKRIVAIN VPKWTNLMHL DTWLTMLDKD KFLYSPIAND VFKFWDYDLV NGGAEPQPVE NGLPLEGLLQ SIINKKPVLI PIAGEGASQM EIERETHFDG TNYLAIRPGV VIGYSRNEKT NAALEAAGIK VLPFHGNQLS LGMGNARCMS MPLSRKDVKW

Produktmerkmale: Biological activity was measured by its ability to induce apoptosis in Jurkat cells using a concentration of 100-150 ng/mL.

Endotoxin-Niveau: Endotoxin level is less than 0.1 ng per μg (1 EU/μg).

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Handhabung: As with any protein, exposing Mycoplasma Arginine Deiminase recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Lagerung: -20 °C

Informationen zur Lagerung: The recombinant protein is stable for at least 2 years from date of receipt at -20 °C. Reconstituted Mycoplasma Arginine Deiminase stable for at least 3 months when stored in working aliquots with a carrier protein at -20 °C.

Haltbarkeit: 24 months

Antigendetails

Target: Mycoplasma Arginine Deiminase

Hintergrund: Arginine Deiminase (ADI) is a microbial enzyme from Mycoplasma produced in E.coli. It has high affinity to L-arginine and hydrolyzes L-arginine to citrulline and ammonia. Low concentrations of ADI have been shown to inhibit proliferation in certain cultured cells by arresting the cell cycle in G (1) and/or S phase. Higher concentrations of ADI lead to subsequent apoptosis. Recombinant Mycoplasma Arginine Deiminase is a 46.4 kDa protein consisting of 409 amino acids.

Gen-ID: 8970640

NCBI Accession: YP_003560236

OMIM: 294155852

UniProt: D5E643