Taq RecA (Active) Protein

Produktnummer ABIN2452211

Produktdetails

Target: Taq RecA

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Thermus aquaticus

Quelle: Escherichia coli (E. coli)

Applikation: Functional Studies (Func)

Produktmerkmale: Single-strand dependent ATPase activity. The activity of single-stranded DNA-dependent ATPase was confirmed.

Reinheit: > 90 % of protein determined by SDS-PAGE (CBB staining)

Anwendungsinformationen

Applikationshinweise: 1) Useful for studying homologous recombination
2) Increase the specificity and yield of multiplex PCR (of cDNA or genomic DNA) by promoting homologous annealing of primers to target DNA
3) Visualization of DNA with electoron microscopy due to nucleofilament formation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 50 mM Tris-HCl ( pH 8.0), 200 mM NaCl, 1 mM EDTA, 50 % glycerol

Lagerung: -20 °C

Referenzen

Shigemori, Mikawa, Shibata, Oishi: "Multiplex PCR: use of heat-stable Thermus thermophilus RecA protein to minimize non-specific PCR products." in: Nucleic acids research, Vol. 33, Issue 14, pp. e126, (2005) (PubMed).

Angov, Camerini-Otero: "The recA gene from the thermophile Thermus aquaticus YT-1: cloning, expression, and characterization." in: Journal of bacteriology, Vol. 176, Issue 5, pp. 1405-12, (1994) (PubMed).

Antigendetails

Target: Taq RecA

Hintergrund: Thermus aquaticus RecA protein is a thermostable enzyme which plays important roles in homologous recombination and DNA repair. This protein has activities of single-stranded DNA dependent ATPase, DNA annealing, and exchanging of strands between two recombining DNA double helices, similar to E.coli RecA protein, but the optimal temperature is between 65~75 °C. Taq RecA was expressed in E.coli in large quantities and the protein was highly purified. MW is 36.5kD.

UniProt: P48296