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T4 SSB (Active) Protein

Spezies: Bacteriophage T4 Wirt: Escherichia coli (E. coli) Recombinant > 95 % of protein determined by SDS-PAGE (CBB staining) Func Active
Produktnummer ABIN2452207
  • Target
    T4 SSB
    Protein-Typ
    Recombinant
    Biologische Aktivität
    Active
    Spezies
    Bacteriophage T4
    Quelle
    Escherichia coli (E. coli)
    Applikation
    Functional Studies (Func)
    Produktmerkmale
    The absence of endonucleases and exonucleases was confirmed.
    Reinheit
    > 95 % of protein determined by SDS-PAGE (CBB staining)
  • Applikationshinweise
    1. Promoting DNA replication and recombination by stabilizing single-stranded DNA
    2. Increase specificity and yields of long PCR products
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    10 mg/mL
    Buffer
    20 mM Tris-HCl ( pH 8.0), 100 mM NaCl, 0.5 mM dithiothreitol, 1 mM EDTA, 50 % glycerol
    Konservierungsmittel
    Dithiothreitol (DTT)
    Vorsichtsmaßnahmen
    This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
  • Bittner, Burke, Alberts: "Purification of the T4 gene 32 protein free from detectable deoxyribonuclease activities." in: The Journal of biological chemistry, Vol. 254, Issue 19, pp. 9565-72, (1979) (PubMed).

    Alberts, Frey: "T4 bacteriophage gene 32: a structural protein in the replication and recombination of DNA." in: Nature, Vol. 227, Issue 5265, pp. 1313-8, (1970) (PubMed).

  • Target
    T4 SSB
    Substanzklasse
    Phage Protein
    Hintergrund
    T4 gene 32 protein is a single-stranded DNA binding protein from phage T4 which binds to single-stranded DNA with high specificity. It is involved in DNA replication and recombination. The T4 phage-derived SSB gene was expressed in E.coli and the protein was highly purified. MW is 33.5 kDa.
    UniProt
    P03695
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