T4 SSB (Active) Protein

Produktnummer ABIN2452207

Recombinant -Protein exprimiert in Escherichia coli (E. coli) und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für T4 SSB (Active) Protein (ABIN2452207)

Target: T4 SSB

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Bacteriophage T4

Quelle: Escherichia coli (E. coli)

Applikation: Functional Studies (Func)

Reinheit: > 95 % of protein determined by SDS-PAGE (CBB staining)

Produktdetails

Produktmerkmale: The absence of endonucleases and exonucleases was confirmed.

Anwendungsinformationen

Applikationshinweise: 1. Promoting DNA replication and recombination by stabilizing single-stranded DNA
2. Increase specificity and yields of long PCR products

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL

Buffer: 20 mM Tris-HCl ( pH 8.0), 100 mM NaCl, 0.5 mM dithiothreitol, 1 mM EDTA, 50 % glycerol

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Referenzen

Bittner, Burke, Alberts: "Purification of the T4 gene 32 protein free from detectable deoxyribonuclease activities." in: The Journal of biological chemistry, Vol. 254, Issue 19, pp. 9565-72, (1979) (PubMed).

Alberts, Frey: "T4 bacteriophage gene 32: a structural protein in the replication and recombination of DNA." in: Nature, Vol. 227, Issue 5265, pp. 1313-8, (1970) (PubMed).

Antigendetails

Target: T4 SSB

Substanzklasse: Phage Protein

Hintergrund: T4 gene 32 protein is a single-stranded DNA binding protein from phage T4 which binds to single-stranded DNA with high specificity. It is involved in DNA replication and recombination. The T4 phage-derived SSB gene was expressed in E.coli and the protein was highly purified. MW is 33.5 kDa.

UniProt: P03695