HIV-1 Reverse Transcriptase (HIV1RT) (Active) Protein

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Proteinname
Spezies
Human Immunodeficiency Virus (HIV)
2
Quelle
Escherichia coli (E. coli)
2
Protein-Typ
Recombinant
Biologische Aktivität
Active
Applikation
Dot Blot (DB), Functional Studies (Func), SDS-PAGE (SDS), ELISA, Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Produktmerkmale Activity: 5,000 units/mL
Reinheit > 90 % by SDS-PAGE (CBB staining)
Hintergrund HIV-1 reverse transcriptase is an RNA-dependent DNA polymerase derived from HIV-1 (AIDS virus), subtype B origin. It also has RNaseH activity and is an enzyme indispensable for reproduction of AIDS virus. This protein is uniquely over-expressed as a recombinant protein in E. coli by a patented method and highly purified. It is composed of two subunits whose molecular weights are 66 kD and 51 kD, same as the those of the enzyme purified from AIDS virus particles.
Applikations-hinweise 1) It is extremely effective for screening new specific inhibitors for HIV virus as a drug for treating AIDS.
2) Generally,Gag and Env proteins are employed as antigens for detecting anti-HIV-1 antibody. However,by using this enzyme in combination as an antigen,the detection will be more sensitive.
3) Reverse transcriptases are used in the first step of RT-PCR reaction for converting RNA to DNA. The HIV-1 reverse transcriptase can also be applied for RT-PCR method.
4) Standards for SDS-PAGE
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.5 mg/mL
Buffer 50 % glycerol, 40 mM Tris-HCl ( pH 8.3), 50 mM NaCl, 5 mM MgCl2, 0.1 % Triton X-100, 1 mM DTT
Konservierungs-mittel Dithiothreitol (DTT)
Vorsichtsmaßnahmen This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Bilder des Herstellers
SDS-PAGE (SDS) image for HIV-1 Reverse Transcriptase (HIV1RT) (Active) protein (ABIN2452197) HIV-1 Reverse Transcriptase (HIV1RT) (Active) protein
Western Blotting (WB) image for HIV-1 Reverse Transcriptase (HIV1RT) (Active) protein (ABIN2452197) HIV-1 Reverse Transcriptase (HIV1RT) (Active) protein (Image 2)
SDS-PAGE (SDS) image for HIV-1 Reverse Transcriptase (HIV1RT) (Active) protein (ABIN2452197) HIV-1 Reverse Transcriptase (HIV1RT) (Active) protein (Image 3)
SDS-PAGE (SDS) image for HIV-1 Reverse Transcriptase (HIV1RT) (Active) protein (ABIN2452197) HIV-1 Reverse Transcriptase (HIV1RT) (Active) protein (Image 4)
Produkt verwendet in: Saitoh, Iwasaki, Nakata, Adachi, Shinagawa: "Overproduction of human immunodeficiency virus type I reverse transcriptase in Escherichia coli and purification of the enzyme." in: Microbiology and immunology, Vol. 34, Issue 6, pp. 509-21, 1990 (PubMed).

Adachi, Gendelman, Koenig, Folks, Willey, Rabson, Martin: "Production of acquired immunodeficiency syndrome-associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone." in: Journal of virology, Vol. 59, Issue 2, pp. 284-91, 1986 (PubMed).

Holtermüller, Bohlen, Castro, Weis: "[Considerations on antacid therapy]." in: Medizinische Klinik, Vol. 72, Issue 30, pp. 1229-41, 1977 (PubMed).