RecA (Active) Protein

Produktnummer ABIN2452179

Produktdetails

Target: RecA

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: E. coli

Quelle: Escherichia coli (E. coli)

Applikation: Functional Studies (Func)

Produktmerkmale: The product is over-expressed as a recombinant protein and highly purified by several steps of chromatography. A single band is observed by SDS-PAGE at 38 kD.

Reinheit: > 90 % by SDS-PAGE (CBB staining)

Anwendungsinformationen

Applikationshinweise: 1) Studies on homologous recombination mechanism and SOS response.
2) Useful in the screening using probe from library by promotion of DNA hybridization (2).
3) Facilitate DNA observation by electron microscope due to nucleofilament formation with DNA.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.6 mg/mL

Buffer: 20 mM Tris-HCl ( pH 8.0), 1 mM EDTA, 150 mM KCl, 1 mM DTT, 50 % glycerol

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20 C (For long term storage: -70 C)

Referenzen

Walker: "Understanding the complexity of an organism's responses to DNA damage." in: Cold Spring Harbor symposia on quantitative biology, Vol. 65, pp. 1-10, (2003) (PubMed).

Taidi-Laskowski, Tyan, Honigberg, Radding, Grumet: "Use of RecA protein to enrich for homologous genes in a genomic library." in: Nucleic acids research, Vol. 16, Issue 16, pp. 8157-69, (1988) (PubMed).

Antigendetails

Target: RecA

Hintergrund: E. coli RecA protein is a very important enzyme for homologous recombination and recombinational repair. Its synthesis is induced by SOS response caused by DNA damage. RecA protein has multiple functions such as single stranded DNA dependent ATPase activity, DNA annealing activity, formation of D-loop and Holliday structure in homologous recombination reaction, and coprotease activities that promote self-cleavages of LexA repressor, lambda phage repressor and UmuD protein. RecA protein binds to single and double stranded DNA for nucleofilament formation. It carries out a central role in homologous recombination. Its homologs in eukaryotes are Rad51 protein and Dmcl protein.

UniProt: P0A7G6