RecA (Active) Protein

Details zu Produkt Nr. ABIN2452179, Anbieter: Anmelden zum Anzeigen
Proteinname
Spezies
Escherichia coli (E. coli)
Quelle
Escherichia coli (E. coli)
Protein-Typ
Recombinant
Biologische Aktivität
Active
Applikation
Functional Studies (Func)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Produktmerkmale The product is over-expressed as a recombinant protein and highly purified by several steps of chromatography. A single band is observed by SDS-PAGE at 38 kD.
Reinheit > 90 % by SDS-PAGE (CBB staining)
Proteinname
Hintergrund E. coli RecA protein is a very important enzyme for homologous recombination and recombinational repair. Its synthesis is induced by SOS response caused by DNA damage. RecA protein has multiple functions such as single stranded DNA dependent ATPase activity, DNA annealing activity, formation of D-loop and Holliday structure in homologous recombination reaction, and coprotease activities that promote self-cleavages of LexA repressor, lambda phage repressor and UmuD protein. RecA protein binds to single and double stranded DNA for nucleofilament formation. It carries out a central role in homologous recombination. Its homologs in eukaryotes are Rad51 protein and Dmcl protein.
UniProt P0A7G6
Applikations-hinweise 1) Studies on homologous recombination mechanism and SOS response.
2) Useful in the screening using probe from library by promotion of DNA hybridization (2).
3) Facilitate DNA observation by electron microscope due to nucleofilament formation with DNA.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1.6 mg/mL
Buffer 20 mM Tris-HCl ( pH 8.0), 1 mM EDTA, 150 mM KCl, 1 mM DTT, 50 % glycerol
Konservierungs-mittel Dithiothreitol (DTT)
Vorsichtsmaßnahmen This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung -20 C (For long term storage: -70 C)
Bilder des Herstellers
SDS-PAGE (SDS) image for RecA (Active) protein (ABIN2452179) RecA (Active) protein
SDS-PAGE (SDS) image for RecA (Active) protein (ABIN2452179) RecA (Active) protein (Image 2)
SDS-PAGE (SDS) image for RecA (Active) protein (ABIN2452179) RecA (Active) protein (Image 3)
Produkt verwendet in: Walker: "Understanding the complexity of an organism's responses to DNA damage." in: Cold Spring Harbor symposia on quantitative biology, Vol. 65, pp. 1-10, 2003 (PubMed).

Taidi-Laskowski, Tyan, Honigberg, Radding, Grumet: "Use of RecA protein to enrich for homologous genes in a genomic library." in: Nucleic acids research, Vol. 16, Issue 16, pp. 8157-69, 1988 (PubMed).

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