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RNASEH Protein

Dieses Recombinant RNASEH-Protein wird in Escherichia coli (E. coli) exprimiert und wurde in 3+ Publikationen erwähnt.
Produktnummer ABIN3200996

Kurzübersicht für RNASEH Protein (ABIN3200996)

Target

Alle RNASEH Proteine anzeigen
RNASEH (Ribonuclease H (RNASEH))

Protein-Typ

Recombinant

Biologische Aktivität

Active

Spezies

  • 2
  • 1
  • 1
E. coli

Quelle

  • 4
Escherichia coli (E. coli)

Reinheit

> 95 % protein determined by SDS-PAGE (CBB staining)
  • Produktmerkmale

    Specific Activity: 100, 000 units/mg protein
    Unit Definition: 1 unit is defined as the amount of the enzyme that hydrolyzes 1 nmol of the RNA in 3H labeled M13 DNA/RNA hybrid to acid-soluble ribonucleotides in 20 min at 37 C.
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  • Applikationshinweise

    1) Removal of mRNA in DNA/RNA hybrid prior to the synthesis of the second strand of cDNA.
    2) Removal of poly (A) tails from mRNA after hybridization with oligo (dT)
    3) Oligodeoxyribonucleotide-directed site-specific cleavage of RNA

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    50 units/L

    Buffer

    50 units/ul in 20 mM Tris-HCl ( pH 7.5), 100 mM KCl, 1 mM DTT, 50 % Glycerol

    Konservierungsmittel

    Dithiothreitol (DTT)

    Vorsichtsmaßnahmen

    This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    -20 °C
  • Gubler: "Second-strand cDNA synthesis: mRNA fragments as primers." in: Methods in enzymology, Vol. 152, pp. 330-5, (1987) (PubMed).

    Donis-Keller: "Site specific enzymatic cleavage of RNA." in: Nucleic acids research, Vol. 7, Issue 1, pp. 179-92, (1979) (PubMed).

    Vournakis, Efstratiadis, Kafatos: "Electrophoretic patterns of deadenylylated chorion and globin mRNAs." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 72, Issue 8, pp. 2959-63, (1976) (PubMed).

  • Target

    RNASEH (Ribonuclease H (RNASEH))

    Hintergrund

    Ribonuclease H (RNase H) is an endoribonuclease which specifically degrades the RNA strand of an RNA/DNA hybrid, leaving the DNA strand and unhybridized RNA intact. E.coli RNase H (=RNaseHI) was over-expressed in E. coli as a recombinant protein and highly purified. MW is 17.6 kDa.

    UniProt

    P0A7Y4
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