Human Immunodeficiency Virus Surface Glycoprotein (HIV gp120) (AA 34-506) (Active) protein (His tag)

Produktnummer ABIN2181172

Dieses Recombinant -Protein wird in HEK-293 Cells produziert.

Kurzübersicht für Human Immunodeficiency Virus Surface Glycoprotein (HIV gp120) (AA 34-506) (Active) protein (His tag) (ABIN2181172)

Target: Human Immunodeficiency Virus Surface Glycoprotein (HIV gp120)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human Immunodeficiency Virus (HIV)

Quelle: HEK-293 Cells

Reinheit: >95 % as determined by SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 34-506

Aufreinigungstag / Konjugat: His tag

Sequenz: AA 34-506

Produktmerkmale: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 54 kDa. The protein migrates as 65-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C-8 °C), After reconstitution under sterile conditions for 1 month (4 °C-8 °C) or 3 months (-20 °C to -70 °C).

Antigendetails

Target: Human Immunodeficiency Virus Surface Glycoprotein (HIV gp120)

Andere Bezeichnung: GP120 (HIV)

Substanzklasse: Viral Protein

Hintergrund: Human Immunodeficiency Virus (HIV) can be divided into two major types, HIV type 1 (HIV-1) and HIV type 2 (HIV-2). HIV-1 is related to viruses found in chimpanzees and gorillas living in western Africa. HIV-2 is related to viruses found in sooty mangabeys. HIV-1 viruses may be further divided into groups. The HIV-1 group M viruses predominate and are responsible for the AIDS pandemic. Some of the HIV-1 group M subtypes are known to be more virulent or are resistant to different medications. HIV-2 viruses are thought to be less virulent and transmissible than HIV-1 M group viruses. Envelope glycoprotein GP120 (or gp120) is the name of the glycoprotein which forms the spikes sticking out of a HIV virus particle. gp120 is essential for virus entry into cells as it plays a vital role in seeking out specific cell surface receptors for entry. Three gp120s, bound as heterodimers to a transmembrane glycoprotein, gp41, are thought to combine in a trimer to form the envelope spike, which is involved in virus-cell attachment. One half of the molecular weight of gp120 is due to the carbohydrate side chains (the "glyco-" in "glycoprotein"). These are sugar residues which form something almost like a sugar "dome" over the gp120 spikes. This dome prevents gp120 from being recognised by the human immune response. As the HIV virus and the human CD4 cell come together, the gp120 binding site "snaps open" at the last minute.The glycoprotein gp120 is anchored to the viral membrane, or envelope, via non-covalent bonds with the transmembrane glycoprotein, gp41. It is involved in entry into cells by binding to CD4 receptors, particularly helper T-cells. Binding to CD4 is mainly electrostatic although there are van der Waals interactions and hydrogen bonds.

Molekulargewicht: 54.1 kDa

Gen-ID: 17