Host-Nuclease Inhibitor Protein Gam (AA 1-138) Protein

Produktnummer ABIN2115469

Dieses Recombinant -Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für Host-Nuclease Inhibitor Protein Gam (AA 1-138) Protein (ABIN2115469)

Target: Host-Nuclease Inhibitor Protein Gam

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 1-138

Verwendungszweck: Recombinant Mouse Sonic Hedgehog/SHH (C25II)

Sequenz: MIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG

Produktmerkmale: Recombinant Bacteriophage Host-nuclease inhibitor protein gam is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Val138) of Bacteriophage GAM fused with a 6His tag at the C-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 1 mM DTT, pH 7.4.

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Lagerung: 4 °C/-20 °C/-80 °C

Informationen zur Lagerung: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: Host-Nuclease Inhibitor Protein Gam

Hintergrund: Host-nuclease inhibitor protein gam(gam) comes from Enterobacteria phage lambda. Originally the gam gene product was assigned to the sequence shown and it was suggested that the gene kil protein is encoded from within this gene and corresponds to residues 41-138. For an alternative assignment of this gene see the entry for the kil gene protein of lambda. The function of the protein inhibits the host recBC-coded nuclease and thus promotes the late, rolling-circle mode of lambda DNA replication.
Synonyms: Host-nuclease inhibitor protein gam,gamma

Molekulargewicht: 19.8 kDa

UniProt: Q62226