Fibronectin Protein (AA 1270-1546, AA 1721-2016)

Produktnummer ABIN2115447

Dieses Recombinant Fibronectin-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für Fibronectin Protein (AA 1270-1546, AA 1721-2016) (ABIN2115447)

Target: Fibronectin

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 1270-1546, AA 1721-2016

Verwendungszweck: Recombinant Human Fibronectin Fragment/RetroNectin/NovoNectin

Sequenz: MPTDLRFTNI GPDTMRVTWA PPPSIDLTNF LVRYSPVKNE EDVAELSISP SDNAVVLTNL LPGTEYVVSV SSVYEQHEST PLRGRQKTGL DSPTGIDFSD ITANSFTVHW IAPRATITGY RIRHHPEHFS GRPREDRVPH SRNSITLTNL TPGTEYVVSI VALNGREESP LLIGQQSTVS DVPRDLEVVA ATPTSLLISW DAPAVTVRYY RITYGETGGN SPVQEFTVPG SKSTATISGL KPGVDYTITV YAVTGRGDSP ASSKPISINY RTEIDKPSMA IPAPTDLKFT QVTPTSLSAQ WTPPNVQLTG YRVRVTPKEK TGPMKEINLA PDSSSVVVSG LMVATKYEVS VYALKDTLTS RPAQGVVTTL ENVSPPRRAR VTDATETTIT ISWRTKTETI TGFQVDAVPA NGQTPIQRTI KPDVRSYTIT GLQPGTDYKI YLYTLNDNAR SSPVVIDAST AIDAPSNLRF LATTPNSLLV SWQPPRARIT GYIIKYEKPG SPPREVVPRP RPGVTEATIT GLEPGTEYTI YVIALKNNQK SEPLIGRKKT DELPQLVTLP HPNLHGPEIL DVPST

Produktmerkmale: Recombinant Human Fibronectin fragment/RetroNectin is produced with our E. coli expression system.The target protein is expressed with sequence (Pro1270-Ser1546&Ala1721-Thr2016) of Human Fibronectin.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Handhabung: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Lagerung: 4 °C/-20 °C/-80 °C

Informationen zur Lagerung: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: Fibronectin

Hintergrund: "Fibronectin1(FN1) is a secreted protein and contains 12 fibronectin type-I domains, fibronectin type-II domains and 16 fibronectin type-III domains.Recombinant human fibronectin fragment, is a protein of 63 kDa containing a central cell-binding domain (type III repeat, 8, 9, 10), a high affinity heparin-binding domain II (type III repeat, 12, 13, 14), and CS1 site within the alternatively spliced III CS region of human fibronectin. Cells bind to a VLA-4 ligand, a CS-I site, and a VLA-5 ligand, a cell attachment domain, and virus vectors binds to a heparin binding domain II, which co-locates the cell and the virus vector on RetroNectin. "
Synonyms: Fibronectin, Retronectin, CIG, FN, Cold-insoluble globulin

Molekulargewicht: 62.7 kDa

UniProt: P02751