Heregulin1-Beta1(HRG1-Beta1) (AA 177-241), (Isoform 6) (Active) Protein

Produktnummer ABIN2017956

Produktdetails

Target: Heregulin1-Beta1(HRG1-Beta1)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: Isoform 6, AA 177-241

Spezies: Human

Quelle: Escherichia coli (E. coli)

Produktmerkmale: ED50 < 1 ng/mL, measured by a cell proliferation assay using MCF-7 cells, corresponding to a specific activity of > 1x 10^6 units/mg.
AA 177-241 (isoform 6), expressed with an N-terminal Met.

Reinheit: > 95 % by SDS-PAGE analysis.

Endotoxin-Niveau: < 0.2 EU/μg, determined by LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstituted in ddH2O at 100 μg/mL.

Buffer: Lyophilized after extensive dialysis against PBS.

Lagerung: -80 °C

Informationen zur Lagerung: Lyophilized recombinant human Heregulin1-Beta1(HRG1-Beta1) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rhHRG1-Beta1 should be stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.

Haltbarkeit: 6 months

Referenzen

Hedayatizadeh-Omran, Valadan, Rafiei, Tehrani, Alizadeh-Navaei: "VERO Stable Cell Lines Expressing Full-Length Human Epidermal Growth Factor Receptors 2 and 3: Platforms for Subtractive Phage Display." in: DNA and cell biology, Vol. 34, Issue 9, pp. 573-8, (2015) (PubMed).

Antigendetails

Target: Heregulin1-Beta1(HRG1-Beta1)

Hintergrund: Heregulin1-Beta1(HRG1-Beta1) is one of the isoforms encoded by Neuregulin (NRG) genes. NRGs are synthesized as large transmembrane precursor proteins, and the NRG family has 4 members and 26 isoforms. These isoforms provide large diversities, including different tissue distribution, variable potencies, and different biological functions. HRG1-beta1 belongs to Type I HRG1, and is expressed in neural tissue, respiratory epithelia, and heart. In vivo, HRG1 binds and activates both ErbB3 and ErbB4, the transmembrane receptor tyrosine kinase, and is involved in the proliferation, differentiation, and survival of cells. Aberrantly produced HRG1 could be used in the constitute activation of the ErbB receptors, therefore, the upregulation of HRG1 contributes to the development of tumors, including breast cancer.Recombinant human Heregulin1-Beta1(HRG1-Beta1) produced in E. coli is a single non-glycosylated polypeptide chain containing 66 amino acids. A fully biologically active molecule, rh HRG1-Beta1 has a molecular mass of 7.6 kDa analyzed by reducing SDS-PAGE.
Synonyms: Neuregulin1 (NRG1-beta 1)

Molekulargewicht: 7.6 kDa, observed by reducing SDS-PAGE.

UniProt: Q02297