TMPRSS15 Protein

Produktnummer ABIN2017742

Dieses Recombinant TMPRSS15-Protein wird in Yeast (Pichia pastoris) produziert.

Kurzübersicht für TMPRSS15 Protein (ABIN2017742)

Target: TMPRSS15 (Transmembrane Protease, serine 15 (TMPRSS15))

Protein-Typ: Recombinant

Spezies: Rind (Kuh)

Quelle: Yeast (Pichia pastoris)

Reinheit: > 95 % by SDS-PAGE analyses.

Produktdetails

Produktmerkmale: 5 IU/μl
Unit Definition: One unit is defined as the amount of enzyme needed to cleave 50 μg of fusion protein in 16 hours to 95 % completion at 22 °C in a buffer containing 25 mM Tris-HCl, pH 8.0.

Sterilität: Sterile

Endotoxin-Niveau: < 1.0 EU/μg, determined by LAL method.

Bestandteile: 100 IU (or 500IU or 5000IU) Recombinant Bovine Enterokinase (in 20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl2, 50% glycerol)
100 μg Cleavage Control Protein (Lyophilized after extensive dialysis against PBS, pH 7.0)
3.6 ml EK Dilution/Storage Buffer (20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl2, 50% glycerol)
1.8 ml 10X EK Cleavage/Capture Buffer (200mM Tris-HCl, pH 7.4, 500mM NaCl, 20mM CaCl2) Storage: Store kit components at -20 °C.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Sterile liquid solution contians 20 mM Tris, 200 mM NaCl, 2 mM CaCl2, 50 % glycerol, pH 7.4.

Handhabung: Avoid freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Recombinant Bovine Enterokinase (rbEK) remains stable up to 1 year at -20 °C from date of receipt. It will remain stable at 37 °C for one week without losing any activity.

Haltbarkeit: 12 months

Antigendetails

Target: TMPRSS15 (Transmembrane Protease, serine 15 (TMPRSS15))

Andere Bezeichnung: Enterokinase(EK)

Hintergrund: Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by a proline. Recombinant Bovine Enterokinase (rbEK) as the light chain is a single glycosylated polypeptide chain containing 200 amino acids. A fully biologically active molecule, rbEK has a molecular mass of 22.7 kDa and is obtained by proprietary chromatographic techniques.
Synonyms: Enteropeptidase, ENTK, PRSS7

Molekulargewicht: Theoretical MW: 22.7 kDa.
Apparent MW: 40.0 kDa, observed by reducing SDS-PAGE.