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Nitrogenase Protein (Nifh)

Dieses Nitrogenase-Protein wird in exprimiert und wurde in 1 Publikation erwähnt.
Produktnummer ABIN190740

Kurzübersicht für Nitrogenase Protein (Nifh) (ABIN190740)

Target

Nitrogenase (Nifh)

Wirt

Bitte anfragen

Applikation

Western Blotting (WB)
  • Marke

    SERVA®

    Aufreinigung

    affinity purified
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  • Applikationshinweise

    Standard curve: 3 loads are recommended (0.5, 2 and 4 i1/4l).For most applications a sample load of 0.2 i1/4g of chlorophyll will give a NifH signal in this range.Positive control: a 2 i1/4l load per well is optimal for most chemiluminescent detection systems. This standard is stabilized and ready and does not require heating before loading on the gel.Please note that this product contains 10 % glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.

    Kommentare

    Concentration: after adding 225 μl of milliQ water final concentration of this standard is 0.15 pmoles/ul and this reagent is ready to use and load on a gel.Protein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.This standard is ready-to-load and does not require any additions or heating. It needs to be fully thawed and thoroughly mixed prior to using. Avoid vigorous vortexing, as buffers contain detergent. Following mixing, briefly pulse in a microcentrifuge to collect material from cap.This standard is stabilized and ready and does not require heating before loading on the gel. Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    For reconstitution add 225 μL of milliQ water

    Buffer

    Glycerol 10 %, Tris Base 141 mM, Tris HCl 106 mM, LDS 2 %, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1 mg/mL PefaBloc protease inhibitor (Roche), 50 mM DTT. This standard is ready-to-load and does not require any additions or heating.

    Handhabung

    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Levitan, Brown, Sudhaus, Campbell, LaRoche, Berman-Frank: "Regulation of nitrogen metabolism in the marine diazotroph Trichodesmium IMS101 under varying temperatures and atmospheric CO2 concentrations." in: Environmental microbiology, Vol. 12, Issue 7, pp. 1899-912, (2010) (PubMed).

  • Target

    Nitrogenase (Nifh)

    Hintergrund

    Nitrogenase is involved in biological fixation of nitrogen to assimilable ammonia.This product is a recombinant protein standard, source: Nostoc/Anabaena 7120.

    Molekulargewicht

    34 kDa (larger than a native protein due to the addition of His-tag)
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