UBE2M Protein (AA 1-183)
Kurzübersicht für UBE2M Protein (AA 1-183) (ABIN1692405)
Target
Alle UBE2M (ube2m) Proteine anzeigenProtein-Typ
Spezies
Quelle
Reinheit
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Proteineigenschaft
- AA 1-183
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Verwendungszweck
- Recombinant Human NEDD8-Conjugating Enzyme UBC12/UBE2M
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Sequenz
- MIKLFSLKQQ KKEEESAGGT KGSSKKASAA QLRIQKDINE LNLPKTCDIS FSDPDDLLNF KLVICPDEGF YKSGKFVFSF KVGQGYPHDP PKVKCETMVY HPNIDLEGNV CLNILREDWK PVLTINSIIY GLQYLFLEPN PEDPLNKEAA EVLQNNRRLF EQNVQRSMRG GYIGSTYFER CLK
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Produktmerkmale
- Recombinant Human NEDD8-conjugating enzyme Ubc12/UBE2M is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Lys183) of Human UBE2M.
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Sterilität
- 0.2 μm filtered
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Endotoxin-Niveau
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Rekonstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
Buffer
- Supplied as a 0.2 μm filtered solution of 50 mM HEPES, pH 7.5,2 mM DTT,150 mM NaCl,10 % glycerol .
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Konservierungsmittel
- Dithiothreitol (DTT)
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Vorsichtsmaßnahmen
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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Lagerung
- -80 °C
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Informationen zur Lagerung
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. -
Haltbarkeit
- 6 months
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- UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))
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Andere Bezeichnung
- NEDD8-Conjugating Enzyme Ubc12/UBE2M
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Hintergrund
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UBE2M is a member of the E2 ubiquitin-conjugating enzyme family. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. UBE2M accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. It involved in cell proliferation and is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation.
Alternative Names: NEDD8-conjugating enzyme Ubc12, NEDD8 carrier protein, NEDD8 protein ligase, Ubiquitin-conjugating enzyme E2 M, UBC12, UBE2M, -
Molekulargewicht
- 20.9 kDa
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UniProt
- P61081
Target
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