AHSA1 Protein

Produktnummer ABIN1686715

Dieses Recombinant AHSA1-Protein wird in Escherichia coli (E. coli) exprimiert und wurde in 1 Publikation erwähnt.

Kurzübersicht für AHSA1 Protein (ABIN1686715)

Target: AHSA1 (Activator of HSP90 ATPase Activity 1 (AHSA1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Western Blotting (WB), SDS-PAGE (SDS), Functional Studies (Func)

Reinheit: >90%

Produktdetails

Sequenz: SHMAKWGEGD PRWIVEERAD ATNVNNWHWT ERDASNWSTD KLKTLFLAVQ VQNEEGKCEV TEVSKLDGEA SINNRKGKLI FFYEWSVKLN WTGTSKSGVQ YKGHVEIPNL SDENSVDEVE ISVSLAKDEP DTNLVALMKE EGVKLLREAM GIYISTLKTE FTQGMILPTM NGESVDPVGQ PALKTEERKA KPAPSKTQAR PVGVKIPTCK ITLKETFLTS PEELYRVFTT QELVQAFTHA PATLEADRGG KFHMVDGNVS GEFTDLVPEK HIVMKWRFKS WPEGHFATIT LTFIDKNGET ELCMEGRGIP APEEERTRQG WQRYYFEGIK QTFGYGARLF

Spezifität: ~38 kDa

Produktmerkmale: 2 µM ABIN1686714 generated a 9-fold ATPase activation of 2 µM Hsp90 (His-tagged Hsp90 beta) in 33 mM Hepes pH 7.2, 30 mM NaCl, 5 mM MgCl2, 1 mM DTT, 1.5 mM ATP in a 100 µL reaction at 37 degrees C. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm.)

Aufreinigung: Multi-Step Purified

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

This product has been certified >90% pure using SDS PAGE analysis. 2uM ABIN1686714 generated a 9-fold ATPase activation of 2uM HSP90 (His-tagged HSP90 beta) in 33mM Hepes pH7.2, 30mM NaCl, 5mM MgCl2, 1mM DTT, 1.5mM ATP in a 100ul reaction at 37 degrees C. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm.)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: Lot specific

Buffer: 20 mM HEPES buffer pH 7.2, 80 mM NaCl, 10 % glycerol

Lagerung: -20 °C

Referenzen

Margarucci, Monti, Cassiano, Mozzicafreddo, Angeletti, Riccio, Tosco, Casapullo: "Chemical proteomics-driven discovery of oleocanthal as an Hsp90 inhibitor." in: Chemical communications (Cambridge, England), Vol. 49, Issue 52, pp. 5844-6, (2013) (PubMed).

Antigendetails

Target: AHSA1 (Activator of HSP90 ATPase Activity 1 (AHSA1))

Andere Bezeichnung: Aha1

Hintergrund: Aha1 is a member of the HSP90 cochaperone family, and is thought to stimulate HSP90 ATPase activity by competing with p23 and other co-chaperones for HSP90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/HSP90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).

Molekulargewicht: approx. 38 kDa

Gen-ID: 10598

NCBI Accession: NP_036243

UniProt: O95433