IL18R1 Protein (C-Term, Extracellular Domain)

Produktnummer ABIN1684708

Dieses Recombinant IL18R1-Protein wird in HEK-293T Cells produziert. Es ist 1 Publikation verfügbar.

Kurzübersicht für IL18R1 Protein (C-Term, Extracellular Domain) (ABIN1684708)

Target: IL18R1 (Interleukin 18 Receptor 1 (IL18R1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: HEK-293T Cells

Reinheit: > 98 %, as determined by SDS-PAGE and HPLC

Produktdetails

Proteineigenschaft: C-Term, Extracellular Domain

Spezifität: Optimized DNA sequence encoding extracellular domain of Mouse IL-18 receptor including an C terminal IgG1 Fc tag was expressed in HEK293 cells.

Produktmerkmale: Recombinant mouse IL-18 receptor alpha is a homodimer protein consisting of two 555 amino acid residue subunits, due to glycosylation migrates as an approximately 95kD protein under reducing SDS-PAGE conditions.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

Anwendungsinformationen

Kommentare:

Biologically active: Measured by its ability to bind immobilized recombinant human IL-18 at a concentration range of 1-32 ng/mL in a functional ELISA assay.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS solution, pH7.2

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: The lyophilized protein is stable for at least years from date of receipt at -20 °C. Upon reconstitution, this cytokine can be stored in working aliquots at - 8 °C for one month, or at -20 °C for six months, with a carrier protein without detectable loss of activity.

Haltbarkeit: 12-24 months

Referenzen

Ebel, Awe, Kaplan, Kansas: "Diverse inflammatory cytokines induce selectin ligand expression on murine CD4 T cells via p38α MAPK." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 12, pp. 5781-8, (2015) (PubMed).

Antigendetails

Target: IL18R1 (Interleukin 18 Receptor 1 (IL18R1))

Andere Bezeichnung: IL18R1

Hintergrund: PDGF is synthesized mainly by megakaryocytes. It is stored in the alpha granules of platelets from which it is released after cell activation of platelets. Platelets synthesize a mixture of the three possible isoforms (BB,AB,AA) while fibroblasts stimulated with EGF synthesize AA homodimers. PDGF receptors are expressed in fibroblasts, osteoblasts, chondroblasts, smooth muscle cells, glial cells, and endothelial cells. Two related receptors, are PDGFRalpha (CD140a) or PDGFRbeta ( CD140b). In contrast to many other cytokines PDGF is not released into the circulation. PDGF binds to several plasma proteins and also to proteins of the extracellular matrix which facilitates local concentration of the factor. The factor functions as a local autocrine and paracrine growth factor. In the adult organism PDGF is involved in wound healing processes. The aberrant expression of PDGF is observed with vascular proliferative diseases such as atherosclerosis. PDGF regulates the synthesis of its own receptor and also influences the expression of membrane receptors for IL1, EGF, 5-Hydroxytryptamine, LDL, transferrin, and muscarinergic receptor.

UniProt: Q61098