CD200 Protein (CD200) (C-Term, Extracellular Domain)

Produktnummer ABIN1684663

Dieses Recombinant CD200-Protein wird in HEK-293T Cells produziert.

Kurzübersicht für CD200 Protein (CD200) (C-Term, Extracellular Domain) (ABIN1684663)

Target: CD200

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: HEK-293T Cells

Reinheit: > 98 %, as determined by SDS-PAGE and HPLC

Produktdetails

Proteineigenschaft: C-Term, Extracellular Domain

Spezifität: Optimized DNA sequence encoding extracellular domain of Mouse CD200 including a C-terminal 6His tag was expressed in HEK293 cells.

Produktmerkmale: Recombinant Mouse CD200 is a monomer protein consisting of13 amino acid residue subunits, due to glycosylation migrates as an approximately 40-45kDa protein on SDS-PAGE.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

Anwendungsinformationen

Kommentare:

The activity was tested by the ability of immobilized recombinant mouse CD200 (1 µg/mL,100 µL/well) to bind mouse CD200R1 with a linear range of 1-200 ng/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS solution, pH7.2

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: The lyophilized antibody is stable for at least 1 year from date of receipt at -20 °C. Upon reconstitution, this antibody can be stored in working aliquots at - 8 °C for one month, or at -20 °C for six months without detectable loss of activity.

Haltbarkeit: 12 months

Antigendetails

Target: CD200

Andere Bezeichnung: CD200

Hintergrund: CD7 is a 40-kD member of the Ig gene superfamily that is expressed on a major subset of human peripheral T lymphocytes and NK cells. CD7 can complex with CD3 and CD45 molecules, and CD7 signaling involves both protein kinase C and protein tyrosine kinase. CD7 has been shown to be a functional signal-transducing molecule on resting NK cells. Although the function of CD7 is unknown, studies suggest that CD7 may be involved in T and NK cell activation and/or adhesion. In TCRbeta T cells, cross-linking of CD7 leads to rapid increases in intracellular calcium concentrations, induces CD7 cytoplasmic domain association with phosphotidylinositol-3-kinase, synergizes with submitogenic amounts of CD3 mAbs to induce T cell activation, modulates T cell adhesion, and increases T cell IL-2 production and IL-2 receptor expression. In TCR T cells, cross-linking of CD7 leads to cell activation and induction of TNF-a, TNF-b, and granulocyte-macrophage CSF mRNA.

UniProt: O54901