PDGF-AB Heterodimer (Homodimer) (Active) Protein

Produktnummer ABIN1589627

Dieses Recombinant PDGF-AB Heterodimer-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für PDGF-AB Heterodimer (Homodimer) (Active) Protein (ABIN1589627)

Target: PDGF-AB Heterodimer

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 % by SDS-PAGE

Produktdetails

Proteineigenschaft: Homodimer

Verwendungszweck: Recombinant Human PDGF-AB Protein

Sequenz: Alpha chain: SIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATTSLNP DYREEDTGRP RESGKKRKRK RLKPT Beta chain: SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT

Spezifität: Chromosomal location:7p22/22q13.1

Produktmerkmale: Length (aa):126/110

Endotoxin-Niveau: < 0.1 ng per ug of PDGF-AB

Anwendungsinformationen

Applikationshinweise: The biological activity was determined by the induction of proliferation in NHDF cells (Normal Human Dermal Fibroblasts).

Kommentare:

Cytokines & Growth Factors

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 50 mM acetic acid,Centrifuge vial prior to opening. The lyophilized PDGF-AB should be reconstituted in 50 mM acetic acid to a concentration not lower than 100 μg/mL. For long term storage of reconstituted protein addition of carrier protein (e.g. BSA or HSA, 0.1 % ) is recommended.

Buffer: 50 mM acetic acid

Handhabung: Avoid repeated freeze-thaw cycles. Centrifuge vial prior to opening

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: The lyophilized PDGF-AB is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted PDGF-AB is best stored at -20°C to -70°C. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 2-3 weeks

Antigendetails

Target: PDGF-AB Heterodimer

Andere Bezeichnung: PDGF-AB

Hintergrund:

Synonyms: PDGFA, PDGF1, PDGF-A

Description: PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-AB is a 25.5 kDa disulfide-linked dimer, consisting of one A chain and one B chains (234 total amino acids).

Molekulargewicht: 25.5 kDa

Gen-ID: 5154, 5155

NCBI Accession: NM_002608, NM_002607, NP_002599, NP_002598

UniProt: P04085