PDGF-AA Homodimer (Homodimer) (Active) Protein

Produktnummer ABIN1589624

Dieses Recombinant -Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für PDGF-AA Homodimer (Homodimer) (Active) Protein (ABIN1589624)

Target: PDGF-AA Homodimer

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 % by SDS-PAGE

Produktdetails

Proteineigenschaft: Homodimer

Verwendungszweck: PDGF-AA

Sequenz: SIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATTSLNP DYREEDTGRP RESGKKRKRK RLKPT

Spezifität: Chromosomal location:7p22

Produktmerkmale: Length (aa):125

Endotoxin-Niveau: < 0.1 ng per ug of PDGF-AA

Anwendungsinformationen

Applikationshinweise: The biological activity was determined by the induction of proliferation in NHDF cells (Normal Human Dermal Fibroblasts).

Kommentare:

Cytokines & Growth Factors

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge vial prior to opening. The lyophilized PDGF-AA should be reconstituted in water to a concentration not lower than 50 μg/mL. For long term storage we recommend to add at least 0.1 % human or bovine serum albumin.

Buffer: 50 mM acetic acid

Handhabung: Centrifuge vial prior to opening. Avoid repeated freeze-thaw cycles.

Lagerung: RT,-20 °C,-80 °C

Informationen zur Lagerung: The lyophilized PDGF-AA is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted PDGF-AA is best stored at -20°C to -70°C. Avoid repeated freeze-thaw cycles.

Antigendetails

Target: PDGF-AA Homodimer

Andere Bezeichnung: PDGF-AA

Hintergrund: PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-AA is a 28.5 kDa disulfide-linked homodimer of two A chains (250 total amino acids).
Synonyms: PDGFA, PDGF1, PDGF-A

Molekulargewicht: 28.5 kDa

Gen-ID: 5154

NCBI Accession: NM_002607, NP_002598

UniProt: P04085