MANF Protein

Produktnummer ABIN1405920

Dieses Recombinant MANF-Protein wird in CHO Cells exprimiert.

Kurzübersicht für MANF Protein (ABIN1405920)

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: CHO Cells

Applikation: Western Blotting (WB), SDS-PAGE (SDS), Cell Culture (CC), In vivo Studies (in vivo)

Produktdetails

Produktmerkmale: Bio-activity testing: Protein is biologically active. Suitable for cell culture and in vivo testing. Human recombinant MANF has been tested in rat &-OHDA model of Parkinson's disease and shown neureprotection and neurorestoration. MANF has also shown neuroprotection in animal models of stroke

Aufreinigung: Purified by ion-exchange chromatography and gel-filtration from serum-free CHO growth media.
Purified Protein is characterized as homogenous material by mass-spectroscopy.

Endotoxin-Niveau: Less than 1EU/µg of protein as determined by LAL method

Anwendungsinformationen

Kommentare:

Synonyms: Mesencephalic astrocyte-derived neurotrophic factor - MANF, ARMET, ARP

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/ml Concentration of the protein is determined by BCA Protein Analysis kit (Pierce). BSA was used as a standard

Buffer: PBS pH 7.4

Handhabung: Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Antigendetails

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Andere Bezeichnung: MANF

Hintergrund: MANF is a trophic factor for midbrain dopamine neurons in vivo. It prevents the 6-OHDA- induced degeneration of dopamine neurons in rodent models of Parkinson’s disease (Lindholm et al., 2008, Voutilainen et al., 2009). When administered after 6-OHDA-lesioning it restores the dopaminergic function and prevents degeneration of dopamine neurons in substantia nigra pars compacta.

Molekulargewicht: Approximately 18.1 kDa, a single non-glycosylated polypeptide chain containing 163 amino acids.

UniProt: P55145