MDH protein (His tag)

Produktnummer ABIN1098249

Dieses Recombinant -Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für MDH protein (His tag) (ABIN1098249)

Target: MDH

Protein-Typ: Recombinant

Spezies: E. coli

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: > 95 % by SDS - PAGE

Produktdetails

Aufreinigungstag / Konjugat: His tag

Aufreinigung: purified by chromatography

Anwendungsinformationen

Kommentare:

Synonyms: malate dehydrogenase, ECK3225, JW3205

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 1 MM DTT, 10% glycerol, 50 mM NaCl

Lagerung: 4 °C

Informationen zur Lagerung: Avoid repeated freezing and thawing cycles.

Antigendetails

Target: MDH

Hintergrund: Malate dehydrogenase (mdh) belongs to the LDH/MDH superfamily and MDH type 1 family. This enzyme catalyzes the conversion of malate into oxaloacetate (using NAD+) and vice versa (this is a reversible reaction). Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules. Recombinant E.coli mdh protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 34.9kDa (336aa), confirmed by MALDI-TOF

NCBI Accession: NP_417703