MMP8 Protein (Catalytic Domain)

Produktnummer ABIN1097894

Dieses Recombinant MMP8-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für MMP8 Protein (Catalytic Domain) (ABIN1097894)

Target: MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Reinheit: > 95% by SDS-PAGE.

Produktdetails

Proteineigenschaft: Catalytic Domain

Sequenz: NPKWER TNLTYRIRNY TPQLSEAEVE RAIKDAFELW SVASPLIFTR ISQGEADINI AFYQRDHGDN SPFDGPNGIL AHAFQPGQGI GGDAHFDAEE TWTNTSANYN LFLVAAHEFG HSLGLAHSSD PGALMYPNYA FRETSNYSLP QDDIDGIQAI YG

Produktmerkmale: Recombinant matrix metalloproteinase-8 (MMP-8, collagenase-2, neurophil collagenase) cloned from human cDNA, expressed in E.coli. The enzyme consists of the catalytic domain of human MMP-8 (residues 105-262)

Aufreinigung: Folding state checked by NMR.

Biological Activity Comment: > 80U/μg. Activity described as U=100 pmol/min at 25°C using a colorimetric assay with thiopeptide Ac-Pro-Leu-Gly-[2-mercapto-4-methyl-pentanoyl]-Leu-Gly-OC2H5 (Biomol) as substrate.

Anwendungsinformationen

Applikationshinweise: Enzyme kinetic studies, cleavage of target substrates and screening of inhibitors

Kommentare:

The enzyme was observed as a single band migrating at a molecular weight of < 20kDa

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.2mg/ml in Tris 20mM pH 7.2, CaCl2 10mM, ZnCl2 0.1mM, NaCl 0.3M, Acetohydroxamic Acid (AHA) 0.5M.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: -80°C. The enzyme is stable at -20°C for at least 1 week. After initial defrost, aliquot enzyme into individual tubes and refreeze at -80°C. Avoid repeated freeze/defrost cycles.

Antigendetails

Target: MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

Andere Bezeichnung: MMP-8

Molekulargewicht: 17.7kDa

UniProt: P22894