IGF-r3 (AA 49-118) (Active) Protein

Produktnummer ABIN1097099

Recombinant -Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für IGF-r3 (AA 49-118) (Active) Protein (ABIN1097099)

Target: IGF-r3

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Functional Studies (Func)

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Produktdetails

Proteineigenschaft: AA 49-118

Verwendungszweck: Recombinant Human LR3 Insulin-Like Growth Factor I/LR3-IGF-1 (MG)

Sequenz: MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA

Produktmerkmale: Recombinant Human LR3-IGF-1 (MG) is produced with our E. coli expression system. The target protein is expressed with sequence (G49-A118) of Human IGF1 R3.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: Less than 0.05 ng/μg (0.5 IEU/μg) as determined by LAL test

Anwendungsinformationen

Kommentare:

Biological activity: ED50 is greater than 200 ng/ml as determined by an IGF binding protein assay.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in 500 mM Acetic Acid.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 300 mM HAc-NaAc, pH 6.5.

Handhabung: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Lagerung: 4 °C/-20 °C/-80 °C

Informationen zur Lagerung: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Haltbarkeit: 3 months

Antigendetails

Target: IGF-r3

Hintergrund: Insulin-like growth factor I (IGF1) belongs to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contains the N- and C-terminal propeptide regions. Mature human IGF-I consisting of 70 amino acids has 94% identity with mouse IGF-I and exhibits cross-species activity. IGF-1 binds IGF-IR, IGF-IIR, and the insulin receptor and plays a key role in cell cycle progression, cell proliferation and tumor progression. IGF-1 expression is regulated by growth hormone. R3 IGF-1 is an 83 amino acid analog of IGF-1 comprising the complete human IGF-1 sequence with the substitution of an Arg (R) for the Glu(E) at position three, hence R3, and a 13 amino acid extension peptide at the N terminus. R3 IGF-1 has been produced with the purpose of increasing biological activity. R3 IGF-1 is significantly more potent than human IGF-I in vitro.
Alternative Names: Insulin-Like Growth Factor I, IGF-I, Mechano Growth Factor, MGF, Somatomedin-C, IGF1, IBP1

Molekulargewicht: 9.1 kDa

UniProt: P05019