IGF-r3 (AA 49-118) (Active) Protein
Spezies: Human
Wirt: Escherichia coli (E. coli)
Recombinant
> 95 % as determined by reducing SDS-PAGE.
Func
Active
-
- Target
- IGF-r3
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 49-118
- Spezies
- Human
- Quelle
- Escherichia coli (E. coli)
- Applikation
- Functional Studies (Func)
- Verwendungszweck
- Recombinant Human LR3 Insulin-Like Growth Factor I/LR3-IGF-1 (MG)
- Sequenz
- MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA
- Produktmerkmale
- Recombinant Human LR3-IGF-1 (MG) is produced with our E. coli expression system. The target protein is expressed with sequence (G49-A118) of Human IGF1 R3.
- Reinheit
- > 95 % as determined by reducing SDS-PAGE.
- Sterilität
- 0.2 μm filtered
- Endotoxin-Niveau
- Less than 0.05 ng/μg (0.5 IEU/μg) as determined by LAL test
-
- Kommentare
-
Biological activity: ED50 is greater than 200 ng/ml as determined by an IGF binding protein assay.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Lyophilized
- Rekonstitution
-
It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in 500 mM Acetic Acid.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 300 mM HAc-NaAc, pH 6.5.
- Handhabung
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Lagerung
- 4 °C/-20 °C/-80 °C
- Informationen zur Lagerung
-
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - Haltbarkeit
- 3 months
-
- Target
- IGF-r3
- Hintergrund
-
Insulin-like growth factor I (IGF1) belongs to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contains the N- and C-terminal propeptide regions. Mature human IGF-I consisting of 70 amino acids has 94% identity with mouse IGF-I and exhibits cross-species activity. IGF-1 binds IGF-IR, IGF-IIR, and the insulin receptor and plays a key role in cell cycle progression, cell proliferation and tumor progression. IGF-1 expression is regulated by growth hormone. R3 IGF-1 is an 83 amino acid analog of IGF-1 comprising the complete human IGF-1 sequence with the substitution of an Arg (R) for the Glu(E) at position three, hence R3, and a 13 amino acid extension peptide at the N terminus. R3 IGF-1 has been produced with the purpose of increasing biological activity. R3 IGF-1 is significantly more potent than human IGF-I in vitro.
Alternative Names: Insulin-Like Growth Factor I, IGF-I, Mechano Growth Factor, MGF, Somatomedin-C, IGF1, IBP1 - Molekulargewicht
- 9.1 kDa
- UniProt
- P05019
-
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