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Protein A Protein (HRP)

Dieses Protein A-Protein wird in exprimiert.
Rockland
Produktnummer ABIN1044879
Hersteller Produkt- Nr.: pa00-03-1000

Kurzübersicht für Protein A Protein (HRP) (ABIN1044879)

Target

Alle Protein A Proteine anzeigen
Protein A

Spezies

Staphylococcus aureus

Wirt

Bitte anfragen

Applikation

ELISA, Western Blotting (WB)
  • Aufreinigungstag / Konjugat

    Dieses Protein A Protein ist gelabelt mit HRP.

    Hersteller Produkt- Nr.

    pa00-03-1000

    Hersteller

    Rockland

    Spezifität

    Protein A Peroxidase was prepared from chromatographically pure Protein A. Protein A Peroxidase was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Protein A. No reaction was observed against anti-Protein G.
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  • Applikationshinweise

    Protein A Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein A Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110). Protein A Peroxidase is stable at 4° C until restored, at which point it is recommended to freeze aliquots at -20° C.

    Kommentare

    Protein A Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein A Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110). Protein A Peroxidase is stable at 4° C until restored, at which point it is recommended to freeze aliquots at -20° C.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

    Konzentration

    1.0 mg/mL

    Buffer

    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Lagerung

    4 °C

    Haltbarkeit

    12 months
  • Target

    Protein A

    Hintergrund

    Protein A is a surface protein (approximately 56kDa in size) originally discovered within the cell wall of Staphylococcus aureus. While important for bacterial survival, Protein A has beneficial uses in immunology for its high affinity binding to immunoglobulins (especially the IgG isotype). This high affinity property makes Protein A essential in the large scale purification of antibodies. Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs).
    Synonyms: HRP, peroxidase, Horseradish peroxidase protein A
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