Potassium Channel KAT3 Antikörper
Kurzübersicht für Potassium Channel KAT3 Antikörper (ABIN488529)
Target
Reaktivität
Wirt
Klonalität
Applikation
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Verwendungszweck
- Anti-KC1 | potassium channel KAT3
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Kreuzreaktivität (Details)
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Arabidopsis thaliana
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
Homologie
- Arabidopsis thaliana
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Aufreinigung
- Immunogen affinity purified serum
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Immunogen
- KLH-conjugated peptide derived from Arabidopsis thaliana KC1 protein P92960
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Applikationshinweise
- 1 : 50 with 125I (WB)
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Kommentare
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In the work of Honsbein et al. 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning. ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems. In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell).
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- For reconstitution add 100 μL of sterile water
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Buffer
- Immunogen affinity purified serum in PBS pH 7.4.
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Handhabung
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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Lagerung
- -20 °C
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Informationen zur Lagerung
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- Potassium Channel KAT3 (KAT3)
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Andere Bezeichnung
- KC1
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Hintergrund
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Background: KC1 is a regulatory K+ channel subunit that assembles with different inward-rectifying K+ channels to affect their activities. The protein is expressed in extremely low amounts, e.g. a few houndred per cell. Alternative names: AKT4, AtKC1, potassium channel TKC
Additional information: For detection images please, refer to the publication belowAntibody detects native and recombinant KC1
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Molekulargewicht
- 75.5 | 75 kDa
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Gen-ID
- 829400
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UniProt
- P92960
Target
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