Purified recombinant S.aureus Protein A protein Expression System: E.coli Molecular weight on SDS-PAGE will appear higher.
Reinheit
> 90 % pure
Endotoxin-Niveau
< 1.0 EU per µg of protein (determined by LAL method)
Applikationshinweise
Protein A protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 10 % glycerol.
Handhabung
Avoid repeated freeze/thaw cycles.
Lagerung
RT/-20 °C
Informationen zur Lagerung
Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
Target
Protein A
Synonyme
alpha protein Peptide, repB Peptide
Hintergrund
Protein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. It binds with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b. Protein A binds with moderate affinity to human IgM, IgA and IgE as well as to mouse IgG3 and IgG1. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology. Recombinant Staphylococcus aureus Protein A was expressed in E. coli and purified by using conventional chromatography. Alternative Names: SPA protein, Immunoglobulin G binding protein A protein, , Immunoglobulin G binding protein A Staphylococcal protein A protein